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| 人脐血间充质干细胞移植对大鼠脊髓损伤后骨密度的影响研究 |
| Effect of transplantation of human umbilical cord blood mesenchymal stem cells on bone mineral density after spinal cord injury in rats |
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| DOI：10.3969/j.issn.1006.7108.2015.12.009 |
| 中文关键词: 脊髓损伤 脐血干间充质细胞 骨密度 |
| 英文关键词:Spinal cord injury Umbilical cord blood mesenchymal stem cells Bone mass density |
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| 中文摘要: |
| 目的 探讨人脐血间充质干细胞移植对大鼠脊髓损伤后骨密度的影响，为脊髓损伤后骨质疏松的临床治疗奠定基础。 方法 建立大鼠脊髓损伤模型，48只SD大鼠随机分为3组：假手术组（Sham)、脊髓损伤组（SCI)、hUCMSCs移植组（SCI + hUCMSCs)，每组16只。分别于术后0周、6周和12周应用双能X线法测定大鼠腰椎及股骨骨密度。结果 0周时,3组之间股骨及腰椎骨密度均无明显差异（P>0. 05) ;6周时，与假手术组比较SCI组及移植组骨密度均明显下降（P<0. 05),而移植组较SCI组有所改善（P<0. 05) ;12周时，与假手术组比较SCI组骨密度明显下降（P <0.01)，移植组骨密度未见明显降低（P >0.05),移植组较SCI组明显改善（P<0.01)。结论 hUCMSCs移植有助于改善大鼠脊髓损伤后股骨及腰椎骨密度,hUCMSCs移植可能用于治疗脊髓损伤骨质疏松，为脊髓损伤后骨质疏松的治疗提供新思路。 |
| 英文摘要: |
| Objective To study the influence of human umbilical cord blood mesenchymal stem cells ( hUCMSCs) transplantation in the changes of bone mineral density (BMD) after complete spinal cord injury (SCI)，and to provide important data for clinical treatment. Methods A total of 48 rats were randomly divided into 3 groups：sham operation group (Sham)，spinal cord injury group (SCI) , and SCI + hUCMSCs group. Sixteen rats were in each group. BMD of the lumbar spine and the femur was measured using dual energy X-ray at 0 week, 6 weeks, and 12 weeks after the operation. Results At 0 week, BMD of the lumbar spine and the femur among the 3 groups was not significantly different (P>0.05). At 6 weeks, BMD decreased significantly in SCI and SCI + hUCMSCs groups compared with sham operation group (P< 0. 05). BMD in SCI + hUCMSCs group was improved compared to that in SCI group (P < 0. 05). At 12 weeks, BMD decreased significantly in SCI group compared with that in sham operation group (P <0. 01), but it was not significant in SCI + hUCMSCs group (P>0. 05), BMD in SCI + hUCMSCs group was improved than that in SCI group (P<0.01). Conclusion Transplantation of hUCMSCs can improve the BMD of the lumbar spine and the femur in rats after complete SCI. It can be used and it provides new idea in the treatment osteoporosis after spinal cord. |
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