| Objective To observe the effect of the Jiangu particles on bone mass, bone micro-structure, and biomechanics in ovariectomized C57 mice using micro CT analysis. Methods Thirty 4-week-old clean grade C57 mice were randomly divided into 3 groups (10 in sham group, and 10 in each of two ovariectomized groups). One week after ovariectomy, mice in two ovariectomized groups were fed with Jiangu particles or saline, respectively. Mice in sham group were fed with normal saline. Two months later, the left tibia was detected using micro CT analysis. The right tibia was detected with 3-point maximal loading. Results Compared to those in sham group, BMC, BMD, Mean, BV, and BS in ovariectomized groups decreased significantly (P<0,01). TMC decreased (0,01 < P< 0. 05). Compared to those in the ovariectomized group, BMC, BMD, and Mean in ovariectomized with Chinese medicine group increased significantly (P <0.01). Image analysis showed that compared to sham group, thin cortical bone in OVX group had a small number of trabecular bone, tiny and discrete distorted fracture shape, or other obvious pathology of osteoporosis. The biomechanical results showed that tibia 3-point maximum load pressure between any two groups was significantly different (P <0. 01). Conclusion Postmenopausal osteoporosis model is successfully established after 2- month ovariectomy. Jiangu particles increase trabecular bone volume and improve the micro-structure and ultimately improve bone strength. Micro CT analysis is simple, efficient, multi-dimensional, and comprehensive in osteoporosis analysis. It has certain advantage comparing to traditional detection methods. |