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| 辅酶Q10对10月龄小鼠股骨的Micro CT观察及骨生物力学检测 |
| Preventive effects of CoQ10 on trabecular microstructure and biomechanical properties of femur in ten-month-old mice with micro CT |
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| DOI：10.3969/j.issn.1006.7108.2016.03.024 |
| 中文关键词: 辅酶Q10；Micro-CT；10月龄 骨生物力学 股骨 |
| 英文关键词:Co Q10 Micro- CT Ten-month-old Bone biomechanical properties Femur |
| 基金项目:国家自然科学基金资助项目（81373499) |
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| 中文摘要: |
| 目的 应用骨生物力学和Micro-CT技术，探讨辅酶Q10对10月龄小鼠股骨的显微结构和生物力学的影响。方法 35 只SPF级8月龄雌性KM小鼠,随机分为正常对照组（CON)、维生素E组（VE)、辅酶Q10低剂量组（Co Q10L)、辅酶Q10高剂量组（Co Q10H）,辅酶Q10低剂量联合维生素E(Co Q10L+VE),连续灌胃给药10周。实验结束后,取右侧股骨进行Micro-CT扫描及三维重建，左侧股骨进行生物力学检测。结果 与CON组比较CoQ10L组小鼠右侧股骨的微观参数：体积分数、连接密度和骨密度明显增加，左侧股骨的骨生物力学刚度增加。Co Q10H体积分数、连接密度和SMI明显增加，同时骨生物力学参数中弹性载荷和最大位移得到明显增加。Co Q10L联合VE组连接密度增加，生物力学方面参数没有统计学差异。与VE比较， 辅酶Q10两个剂量和辅酶Q10低剂量联合维生素E参数并没有统计学差异。结论 COQ10(500 mg/kg?d)能够修复小鼠的股骨微观结构，提高骨生物力学的性能，修复骨量、降低股骨骨折风险的能力优于VE,提示Co Q10的抗骨质疏松作用具有良好的研究前景。 |
| 英文摘要: |
| Aim Bone biomechanics and micro-CT were conducted to investigate the effect of Co-enzyme Q10 on micro-structure and biological mechanics of femur in ten-month-old mice. Methods 35 eight-month-old female KM mice in SPF grade were randomly divided into normal control group (CON) group,vitamin E group(VE), co-enzyme Q10 group(CoQ10L)，co-enzyme Q10 group (CoQ10H)，Co Q10L + VE. Drugs were continuously given for 10 weeks. After the experiment, the femur of right side in each mouse was taken and scanned with micro-CT and 3D-reconstructed. In addition, the left femurs were used to perform bio-mechanical testing. Results Compared with CON group, the microscopic parameters of right femur of mice in CoQ10L group indicated that the bone volume, connection density and bone mineral density were notably increased. Whereas the bone bio-mechanical parameters of left femur showed that the stiffness were markedly increased. CoQ10H group indicated that the bone volume, connection density and SMI were notably increased. Whereas the bone bio-mechanical parameters of left femur showed that maximum displacement and elastic loadwere markedly increased. Co Q10 L + VE group indicated that connection density was notably increased. There was no statistical difference in biomechanical parameters. Compared with VE group, the two doses of Co Q10 and Co Q10L + VE were not significantly different. Conclusion Co Q10(500 mg/kg?d) might repair femoral Micro-structure of mice. The effect of Co Q10 on repairing the bone mass and lowering femoral fracture risk is better than that of VE, implying that the anti-osteoporosis effect of Co Q10 is of promising prospect and should be further explored. |
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