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| 硬化蛋白抗体和跑台运动对大鼠骨代谢的影响 |
| The effect of sclerostin antibody and treadmill exercise on bone metabolism in rats |
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| DOI：10.3969/j.issn.1006.7108.2016.04.007 |
| 中文关键词: 硬化蛋白抗体 跑台运动；骨代谢 大鼠 |
| 英文关键词:Sclerostin antibody Treadmill exercise Bone metabolism Rat |
| 基金项目: |
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| 中文摘要: |
| 目的 通过对糖皮质激素诱导骨质疏松大鼠模型进行硬化蛋白和跑台运动的干预，研究硬化蛋白抗体和跑台运动对大鼠骨代谢的影响。方法 雄性Wistar大鼠饲养到19周后，分为5组：C组（正常对照组）、M组（甲强龙对照组）、M+E组 (甲强龙+运动组）、M+S组（甲强龙+硬化蛋白抗体组）、M+E + S组（甲强龙+运动+硬化蛋白抗体组）。结果（1)注射 甲强龙可以提高脂肪体重和骨细胞凋亡水平，降低全身和股骨骨矿物质含量，降低股骨骨密度和骨陷窝率，且伴随着股骨远端的骨小梁骨量的下降。（2)单纯性的运动可以提高骨量、骨陷窝率，同时可以降低脂肪体重、骨吸收标志物NTx和骨细胞凋亡水平,但运动不会影响骨矿物质含量和皮层骨参数结构。（3)单纯性的注射硬化蛋白抗体能增加骨形成标志物骨钙素 (osteocalcin,OC),防止甲强龙对骨质量的不良影响，进而提高骨含量、骨密度以及骨体积百分比。硬化蛋白抗体可以增加远端和中段部分的皮质骨参数，防止骨陷窝率下降和因甲强龙诱导产生的骨细胞凋亡水平上升。（4)运动和硬化蛋白交互作用时，可以提高骨密度、皮质骨参数,促进骨形成抑制细胞凋亡的发生。结论 单纯性的Scl-Ab注射方案可以预防糖皮质激素治疗对骨量的不良影响，显著提髙皮质骨骨量和骨强度。单纯性的跑台运动方案可以降低脂肪含量，并在不影响皮质骨的情况下防止骨小梁丢失。运动与硬化蛋白之间表现出良好的协同作用，对治疗糖皮质激素诱导骨质疏松症具有积极作用。 |
| 英文摘要: |
| Objective To study the effect of the sclerostin antibody and treadmill exercise on bone metabolism in rats with glucocorticoid-induced osteoporosis. Methods After feeding of 19 weeks, male Wistar rats were divided into 5 groups, group C (normal control group)，group M (with methylprednisolone) , M + E group (with methylprednisolone and exercise), group M + S (with methylprednisolone and sclerostin antibody ) , group M + E + S (with methylprednisolone，exercise，and sclerostin antibody). Results (1) Injection of methylprednisolone improved the weight of body fat and apoptosis bone cells, reduced systemic and femoral bone mineral content, reduced the femoral bone mineral density ( BMD) and bone lacuna, and accompanied by a decrease trabecular bone in the distal femur. (2) Simple exercise improved bone mass，bone lacuna rate, and reduced fat mass and bone resorption marker NTX and apoptosis of bone cells. However, exercise did not affect bone mineral content and cortical bone structure parameters. (3) Simple injection of sclerostin antibody increased bone formation marker osteocalcin, prevented from the adverse effect of methylprednisolone on bone quality, and improved bone mineral content, bone mineral density and bone volume percentage. Sclerostin antibody increased the distal and middle part of the cortical bone parameters and prevented from decrease of bone lacuna and increase of apoptosis due to methylprednisolone. (4) Interaction of exercise and sclerostin antibody increased bone mineral density and the cortical bone parameters，and stimulated bone formation and inhibited apoptosis. Conclusion The simple injection of sclerostin antibody prevents from side effect on bone mass induced by glucocorticoids, and increases cortical bone mass and bone strength. The simple exercise program reduces fat content and prevents trabecular bone loss. The combination of exercise and sclerostin antibody has a positive effect on the treatment of osteoporosis induced by glucocorticoid. |
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