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| 慢性间歇低氧/复氧对大鼠骨代谢的影响 |
| Effect of chronic intermittent hypoxia /reoxygen on bone metabolism in rats |
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| DOI：10.3969/j.issn.1006.7108.2016.05.005 |
| 中文关键词: 慢性间歇低氧/复氧 骨代谢 骨密度 |
| 英文关键词:Intermittent hypoxia/reoxygen Bone metabolism BMD |
| 基金项目:甘肃省自然科学基金项目（1506RJZA253);甘肃省卫计委科研管理项目（GWGL2010-15);甘肃省中医药科研项目（GZK- 2012-39) |
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| 中文摘要: |
| 目的 建立间歇低氧/复氧模型，观察间歇低氧/复氧暴露对SD大鼠骨代谢的影响并探讨其可能的发病机制。方法 将20只8周龄SD雄性大鼠随机分为间歇低氧（IH)组和常氧对照（NC)组，每组10只放入间歇低氧模拟舱进行间歇低氧/复氧造模，8周后处死，测定骨代谢指标骨矿盐含量、血清B-ALP、各部位骨密度（BMD)，氧化应激指标超氧化物歧化酶（SOD)和丙二醛（MDA)水平，炎症反应指标IL-6和TNF-α表达水平。结果 与NC组相比，IH组B-ALP含量升高，全身BMD、股骨 BMD和骨矿盐含量降低，差异均具有统计学意义（P<0.05);SOD水平降低，MDA水平升高，差异均具有统计学意义（P<0.05);IL-6和TNF-α表达水平升高，差异均具有统计学意义（P<0.05)。结论 慢性间歇低氧/复氧条件下可能诱导SD大鼠体内发生氧化应激状态和炎症反应，引起大鼠骨质和相关代谢指标的变化。 |
| 英文摘要: |
| Objective To establish a model of intermittent hypoxia/reoxygen and to observe the effect of exposure to intermittent hypoxia/reoxygen on bone mineral density in SD rats and its possible pathogenesis. Methods Twenty eight-week-old male SD rats were randomly divided into intermittent hypoxia (IH) group and normal oxygen control (NC) group, with 10 rats in each group. Rats were put into the intermittent hypoxia simulation chamber to build intermittent hypoxia/reoxygen model and were killed in 8 weeks. The bone metabolism indexes, including bone mineral content, serum B-ALP, bone mineral density (BMD) of each part, oxidative stress index superoxide dismutase (SOD) and malondialdehyde (MDA) , and inflammation response indicators IL-6 and TNF-α were determined. Results Compared to those in NC group, in IH group B-ALP level increased, but BMD of the whole body and the femur and bone mineral contents decreased, with significant difference (P< 0. 05) ; SOD level decreased but MDA level increased，with significant difference (P < 0. 05);The expression levels of IL-6 and TNF-α increased，with significant difference (P< 0. 05). Conclusion Chronic intermittent hypoxia/reoxygen induces the oxidative stress and inflammatory response in SD rats and the change of bone and related metabolic parameters in rats. |
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