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| 续苓健骨方对骨质疏松模型大鼠骨密度及OPG和RANKL蛋白表达的影响 |
| The effect of Xuling invigorating bone prescription on bone mineral density and the protein expression of OPG and RANKL in rat osteoporosis model |
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| DOI：10.3969/j.issn.1006.7108.2016.05.017 |
| 中文关键词: 中医中药 续苓健骨方 骨密度；OPG RNAKL |
| 英文关键词:Chinese Medicine Xuling invigorating bone prescription Bone mineral density OPG RANKL |
| 基金项目:福建省科技厅省属公益类科研院所基本科研专项 (2014R1035-11 , 2015R1035-11 )。福建省卫生系统中青年骨干人才培养项目 (2015-ZQN-JC-36) |
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| 中文摘要: |
| 目的 研究续苓健骨方对骨质疏松模型大鼠骨密度的作用及其机制。方法 将SD雌性大鼠去卵巢造骨质疏松模型，随机分组：钙尔奇D组、续苓健骨方组和骨质疏松模型组，佐以假手术组对照，给药12周后取材，检测左胫骨骨密度，Elisa方法检测血清骨碱性磷酸酶（BALP)水平，Western blot法测OPG和RANKL蛋白表达。结果（1 )续苓健骨方组骨密度均明显高于其它3组，组间比较有统计学意义（与钙尔奇D组P<0.05，与假手术组P<0.01，与模型组P<0.001)。（2)续苓健骨方组BALP水平明显高于模型组，两组比较有统计学意义，P<0.01，但与假手术组、钙尔奇D组比较无显著性差异，P >0.05。 (3)续苓健骨方组OPG蛋白表达水平明显高于模型组，比较有统计学意义，P<0. 05,RNAKL蛋白表达水平明显低于模型组，比较有统计学意义，P<0.0l。模型组RANKL蛋白表达明显高于假手术组,P<0.05。结论 续苓健骨方能显著提高骨质疏松模型大鼠的骨密度，其机制与上调OPG、下调RNAKL蛋白表达有关。 |
| 英文摘要: |
| Objective To study the effect of Xuling invigorating bone prescription on bone mineral density ( BMD) in rat osteoporosis model. Methods SD female rats were randomly divided into Caltrate D group, Xuling invigorating bone prescription group, osteoporosis model group, and sham-operated control group. Drug administration lasted for 12 weeks. BMD of the left tibia was detected. Serum bone alkaline phosphatase (BALP) level was detected using ELISA method. Protein expression levels of OPG and RANKL were measured using Western blot method. Results (1) BMD was significantly higher in Xuling invigorating bone prescription group than that in other three groups，and the difference between the two groups was statistically significant (compared with Caltrate D group, P <0.05, compared with the sham-operated control, P <0.01, compared with the model group, P < 0.000). (2) BALP was higher in Xuling invigorating bone prescription group than that in osteoporosis model group, and the difference was statistically significant between the two groups (P<0, 01). However, there was no significant difference compare with the sham-operated group and Caltrate D group ( P > 0. 05). (3) OPG protein expression level was higher in Xuling invigorating bone prescription group than that in osteoporosis model group, with statistical significance (P < 0. 05). RANKL protein expression level was lower than in osteoporosis model group, with statistical significance (P < 0. 01). RANKL protein expression level in osteoporosis model group was higher than that in sham-operated control group ( P < 0. 05). Conclusion Xuling invigorating bone prescription significantly improves BMD in osteoporotic rats. The mechanism is related to up-regulation of OPG and down-regulation of RANKL. |
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