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| 替诺福韦酯对小鼠成骨细胞MC3T3-E1增殖、凋亡及矿化的影响 |
| The effect of tenofovir disoproxil fumarate on the proliferation，apoptosis，and mineralization of mouse osteoblast MC3T3E1 cells |
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| DOI：10.3969/j.issn.1006.7108.2016.05.018 |
| 中文关键词: 富马酸替诺福韦酯；成骨细胞 增殖；凋亡 矿化 |
| 英文关键词:Tenofovir disoproxil fumarate Osteoblast Apoptosis Proliferation Mineralization |
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| 中文摘要: |
| 目的 探讨富马酸替诺福韦酯（Tenofovir disoproxil fumarate, TDF)对体外培养小鼠胚胎前体成骨样细胞MC3T3-E1增殖、凋亡及矿化的影响。方法 体外培养MC3T3-E1细胞，分为正常细胞对照组、二甲基亚砜（dimethyl sulfoxide, DMS0)对照组、TDF 处理组（50 nM、500 nM、5000 nM、50000 nM 、250000 nM、500000 nM)，干预 24 h、48 h。cell counting kit-8(CCK8)试剂盒检测细胞增殖抑制率,ANNEXIN V/7AAD凋亡试剂盒检测细胞凋亡率，茜红素染色检测钙结节形成。结果 TDF对MC3T3- E1成骨细胞的增殖和凋亡的影响受TDF浓度和干扰时间的影响，即低浓度对增殖和凋亡无影响，高浓度抑制增殖并促进凋亡，并随干扰时间增加，增殖抑制率和促凋亡率增大，并抑制MC3T3-E1成骨细胞钙结节形成；结论 在血药浓度（约500 nM) 时，TDF对MC3T3-E1成骨细胞增殖和凋亡无影响，但抑制钙结节形成，进而影响成骨，可能是导致TDF相关性骨量减少和骨质疏松的因素之一。 |
| 英文摘要: |
| Objective To investigate the effect of tenofovir disoproxil fumarate (TDF) on proliferation, apoptosis, and mineralization of mouse MC3T3-H1 cells. Methods MC3T3-E1 cells were cultured in vitro，The cells were divided into normal control group, DMSO control group，and TDF treatment groups (50 nM, 500 nM, 5000 nM，50000 nM, 250000 nM，or 500000 nM, respectively). After 24 and 48 hours, the growth inhibitory rate and apoptosis rate were examined using CCK8 kit and ANNEXIN V/7AAD kit, respectively. Calcium nodule was examined using Alizarin red staining. Results The effect of TDF on the proliferation, apoptosis, and mineraiization of MC3T3-E1 ceHs were dose and time-dependent. Low dose of TDF was not effective on the proliferation and apoptosis, but high dose of TDF inhibited the proliferation, promoted apoptosis, and inhibited calcium nodule formation. Conclusion TDF at blood concentration of 500nM is not effective on the proliferation and apoptosis of MC3T3-E1 cells, but it inhibits mineralizationT resulting in TDF-related bone loss and osteoporosis. |
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