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| 补肾强督方对强直性脊柱炎膜内成骨Wnt/β-catenin通路影响的实验研究 |
| Effect of Bushen Qiangdu recipe on Wnt/β-catenin pathway in intramembranous ossification in ankylosing spondylitis |
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| DOI：10.3969/j.issn.1006-7108.2016.06.013 |
| 中文关键词: 强直性脊柱炎 骨化 膜内成骨 Wnt/β-catenin通路 |
| 英文关键词:Ankylosing spondylitis Ossification Intramembranous ossification Wnt/β-catenin pathway |
| 基金项目:北京市自然科学基金青年基金（7144241）；国家自然科学基金面上项目（81373562）；国家自然科学基金青年项目（81403378）；中日友好医院青年科技英才培养计划（2014-QNYC-B-02）
徐愿，孔维萍，为共同第一作者 |
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| 中文摘要: |
| 目的 研究补肾强督方对BMSCs膜内成骨中Wnt通路的作用。方法 BMSCs细胞分为空白对照组、西药对照组、补肾强督方低、中、高剂量组，进行膜内成骨诱导分化，ELISA法检测上清液ALP、BGP，Western blot和荧光定量PCR检测DKK-1和β-catenin蛋白和基因表达。结果 补肾强督方含药血清对BMSCs能促进BMSCs成骨分化中DKK-1蛋白和mRNA表达，抑制β-catenin蛋白和mRNA表达，且呈剂量依赖性，但对成骨能力无影响。结论 补肾强督方能调节BMSCs细胞成骨分化中Wnt通路，可能具有抑制AS骨化的作用。 |
| 英文摘要: |
| Objective To observe the effect of Bushen Qiangdu recipe on Wnt/β-catenin pathway in intramembranous ossification by bone mesenchymal stem cells (BMSCs). Methods BMSCs were divided into the blank control group, the Western medicine group, the low-dose group of Bushen Qiangdu recipe, the middle-dose group of Bushen Qiangdu recipe, and the high-dose group of Bushen Qiangdu recipe. Intramembranous ossification was induced. Enzyme-linked immuno sorbent assay (ELISA) was used to detect supernatant alkaline phosphatase (ALP) and bone gla-protein (BGP). Protein and gene expression of DKK-1 and β-catenin was detected using Western blot and quantitative polymerase chain reaction (PCR). Results Bushen Qiangdu recipe-containing serum promoted the protein and mRNA expression of DKK-1 in osteogenic differentiation of BMSCs, and inhibited the protein and mRNA expression of β-catenin, in a dose-dependent manner. It had no effect on bone formation. Conclusion Bushen Qiangdu recipe regulates Wnt signaling pathway in osteogenic differentiation of BMSCs, and thus may inhibit the ossification in ankylosing spondylitis. |
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