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| 龟甲胶、鹿角胶调控MKK基因表达促进豚鼠OA软骨细胞增殖的研究 |
| The study of tortoise shell glue and deer horn glue regulation of MAPKK gene expression in the promotion of chondrocyte proliferation in guinea pig with osteoarthritis |
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| DOI：10.3969/j.issn.1006.7108.2016.07.003 |
| 中文关键词: 中医中药 骨关节炎 软骨细胞 龟甲胶 鹿角胶 丝裂原活化蛋白激酶激酶 |
| 英文关键词:Traditional Chinese medicine Osteoarthritis Chondrocytes Tortoise shell glue Deer horn glue Mitogen-activated protein kinase kinase (MAPKK) |
| 基金项目:福建省卫生厅中医药科研项目（wzgs201301)，福建省中青年教师教育科研重点项目（科技A类，JA13153) |
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| 中文摘要: |
| 目的 通过观察龟甲胶、鹿角胶含药血清对豚鼠膝骨关节炎软骨细胞增殖及其丝裂原活化蛋白激酶激酶（MKK)基因表达的影响，探讨龟甲胶、鹿角胶对膝骨关节炎的治疗机制。方法 采用Ⅱ型胶原酶消化法获取3月龄豚鼠膝关节软骨细胞，建立体外培养系，将龟甲胶组、鹿角胶组、盐酸氨基葡萄糖组、对照组4组含药血清分别对其进行干预，采用MTT法检测含药血清干预后软骨细胞增殖情况，荧光定量PCR检测含药血清干预对软骨细胞MKK基因表达的影响。结果 5%含药血清干预72 h后，软骨细胞的MTT的检测结果为龟甲胶组（0. 315 ±0. 048)、鹿角胶组（0. 236 ±0. 029)、盐酸氨基葡萄糖组（0. 190 ± 0. 022)、对照组（0. 146 ± 0. 023),差异有统计学意义（P <0.05);荧光定量PCR检测MKK表达量结果为龟甲胶（3. 287 ± 0.675)、鹿角胶（2.147 ±0..204)、盐酸氨基葡萄糖组（1. 137 ± 0. 123)及对照组（0. 627 ± 0. 102 )，差异有统计学意义（P< 0.05)。结论 龟甲胶、鹿角胶对软骨细胞的促增殖作用强于盐酸氨基葡萄糖，这一作用可能与其能有效上调关节软骨细胞 MKK的基因表达有关。 |
| 英文摘要: |
| Objective To explore the mechanism of the treatment of knee osteoarthritis with tortoise shell glue and deer horn glue，by observing the effect of tortoise shell glue and deer horn glue-containing serum on chondrocyte proliferation and mitogen- activated protein kinase kinase (MAPKK) expression in guinea pigs with knee osteoarthritis. Methods Articular chondrocytes were obtained from 3-month-old guinea pig by type II collagenase digestion to establish culture system in vitro. Chondrocytes was additioned with 4 kinds of drug-containing serum respectively (tortoise shell glue group, deer horn glue group, glucosamine hydrochloride group, and the control group). Chondrocyte proliferation was detected using MTT method. MAPKK mRNA expression was detected using quantitative PCR. Results After addition of 5% drug-containing serum for 72 h, chondrocyte proliferation was 0. 315 ±0. 048 in tortoise shell glue group, 0. 236 ±0. 029 in deer horn glue group, 0. 190 ±0. 022 in glucosamine hydrochloride group, and 0. 146 ± 0.023 in the control group, respectively, as tested using MTT method. The difference was statistically significant (P < 0. 05). MAPKK gene expression was 3. 287 ± 0. 675 in tortoise shell glue group，2. 147 ± 0. 204 in deer horn glue group, 1. 137 ± 0. 123 in glucosamine hydrochloride group, and 0. 627 ± 0. 102 in the control group, respectively, as detected using quantitative PCR. The difference was statistically significant (P < 0. 05 ). Conclusion The effect of tortoise shell glue and deer horn glue on proliferation of chondrocytes is stronger than that of glucosamine hydrochloride. The effect may be related to the up-regulation of MAPKK gene expression in articular cartilage cells. |
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