| Objective In order to understand the effects of radiation on osteoblast, the function changes after radiation was investigate by MC3T3-E1 cells. Methods MC3T3-E1 cells were cultured in vitro, and induced into osteoblast precursor and osteoblasts using induction medium. After137Csγ irradiation, the survival of the cells was tested with MTT analysis. The gene expression of RunX2, ALP, and M-CSF was examined using real-time quantitative PCR. Results MTT results showed that with the increase of irradiation doses, the growth rate of normal MC3T3-E1 cells decreased signiticantly, but it was not obvious in induced cells. Real-time quantitative PCR results showed that after 137Csγ irradiation, the gene expression of ALP, RunX2, and M- CSF obviously decreased in MC3T3-E1 cells, but the change was not obvious in the osteogenic precursor cells compared with the normal cells. The gene expression of ALP and Runx2 decreased, and M-CSF showed an increasing trend in differentiated osteoblasts. Conclusion Radiation inhibits the proliferation, development, and differentiation of osteoblasts in early stage. With the differentiation of osteoblasts, the effect of radiation on the proliferation and growth of osteoblasts decreases, but the role of radiation in the osteoblast regulation of osteoclast function is not reduced. |