|
| 辅酶Q10对去卵巢大鼠股骨显微结构及生物力学的影响 |
| Effects of CoQIO on trabecular microstructure and biomechanical properties of femur in ovariectomy rat model |
| |
| DOI：10.3969/j.issn.1006.7108.2016.08.002 |
| 中文关键词: 辅酶Q10 骨生物力学 去卵巢大鼠 股骨 骨质疏松 |
| 英文关键词:CoQ10 Biomechanical properties Ovariectomy rats Femur Osteoporosis |
| 基金项目:国家自然科学基金资助项目（81373499)；广医润和辅酶Q10联合研究中心资助课题（2XX14002) |
|
| 摘要点击次数: 715 |
| 全文下载次数: 605 |
| 中文摘要: |
| 目的 应用骨生物力学和micro-ct技术，探讨CoQ10对去卵巢大鼠的股骨显微结构及生物力学的影响。方法 48只3 月龄SPF级SD雌性未孕大鼠，随机分为假手术组（Sham)、去卵巢模型组（OVX)、小麦胚芽油组（WGO)、WGO +辅酶Q10组 (髙、低剂量）、戊酸雌二醇组（EV),连续给药90d。实验结束后，取右侧股骨进行生物力学检测，然后进行Micro CT扫描及三 维重建。结果 与Sham组比较，OVX组大鼠股骨的微观参数：骨体积分数、骨小梁数量、骨小梁厚度和骨密度明显减少，骨小梁分离度和结构模型指数明显增高；而股骨的骨生物力学参数：最大载荷、断裂载荷及弹性载荷明显减少，刚度明显减少。与OVX组相比，EV组大鼠股骨的骨微观参数均得到了良好地修复，骨生物力学参数中最大载荷、断裂载荷、弹性载荷及刚度得到了明显修复。而小麦胚芽油+低剂量CoQ10组大鼠股骨的微观参数骨小梁数量、骨小梁分离度、结构模型指数及骨密度和骨体积分数则得到一定程度的修复。结论 辅酶Q10(15 mg/kg)能够修复OVX大鼠股骨微观结构破坏，辅酶Q10(15 mg/ kg)可以预防去卵巢手术导致大鼠股骨松质骨结构破坏和股骨生物力学性能的下降，其他剂量组对此无明显的预防作用。提示辅酶Q10的抗骨质疏松作用具有良好的研究前景。 |
| 英文摘要: |
| Objective To investigate the effects of CoQ10 on trabecular microstructure and biomechanical properties of femur in a rat model of bone loss induced by ovariectomy and to compare the effects of CoQIO and Estradiol Valerate. Methods 48 three- month-old female Sprague-Dawley rats were randomly divided into 6 groups (n = 8 per group) which were Sham, OVX, WGO, WGO + CoQ10 (high dose, low dose) and EV. The bone loss model had ovariectomy surgery. The femoral epiphysis end (FED) was analyzed by micro-CT and three-dimensional reconstructions. Results Micro-CT data showed that bone volume/total volume (BV/TV)，trabecular number (Tb. N), trabecular thickness (Tb. Th) and trabecular bone mineral density (BMD) in the OVX group were significantly lower than those in the sham group; whereas the trabecular separation (Tb. Sp) and structure model index (SMI) were significantly greater in the OVX group than those for the sham group. Biomechanical properties including maximum strength, break strength, break strain and stiffness decreased significantly in the OVX group. Compared with the OVX group, the micro-CT data showed that trabecular microstructure was well repaired in the Estradiol Valerate group, with several parameters of the biomechanical properties (maximum strength，break Strength, break strain and stiffness) significantly improved. In the Coql0 group, the biomechanical properties were significantly improved, and several of the micro-CT data (Tb. N, Tb. Sp, SMI and BV/ TV) were well repaired. Conclusion Compared with OVX, CoQ10 could greatly improve the trabecular microstructure as well as the biomechanical properties of femur in a rat model of osteoporosis induced by ovariectomy. CoQ10 may have great potential in the prevention of osteoporosis. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|