| Objective To prepare CIA model and to evaluate the effect of ATI32. Methods CIA model was induced by type II collagen, and the effect of the new drug AT132 was evaluated. The pathological changes of knee and ankle joint were assessed using HE staining. The expression of C II and TNF alpha was detected by ELISA. The expression of TNF alpha, IL-1，IL-6, IL-10 in mice’s synovial was detected using flow cytometry. Results (1) The CIA mice model group showed swelling and deformation of the little finger joints, feet and toes. Mice treated with AT132 showed a slight swelling of joints. (2) The pathology of knee and ankle joints of CIA model mice showed infiltration of inflammatory cells, synovial hyperplasia, appearance of pannus, emergence of osteoclasts, and cartilage and bone destruction. The pathological results of the AT132 group showed a slight inflammatory cell infiltration and synovial hyperplasia. (3) The ELISA results showed that the expression of C II and TNF alpha in the CIA model group increased significantly, whereas the expression of C II and TNF alpha in the AT 132 treated group decreased significantly. (4) Flow cytometry results showed that the expression of TNF alpha, IL-1, IL-6 and IL-10 at the CIA model mice’s synovial increased significantly, whereas the expression of TNF alpha, IL-1, IL-6 and IL-10 of the AT132 treated mice’s synovial decreased significantly. Conclusion The successful rate of the CIA model was 100%. AT132 is effective in the treatment of arthritis in mice. |