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| 低钙日粮诱导蛋鸡骨质疏松模型的研究 |
| The study of hen osteoporosis model using low calcium diet |
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| DOI： |
| 中文关键词: 笼养蛋鸡 骨质疏松 高转化型 碱性磷酸酶 病理切片 |
| 英文关键词:Cage hens Osteoporosis High bone turnover Alkaline phosphatase Pathological slices |
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| 中文摘要: |
| 目的 建立蛋鸡骨质疏松模型，研究蛋鸡骨质疏松，为人类骨质疏松的研究提供参考。方法 用低钙日粮(钙含量
1. 5%)，连续 60d 诱发笼养蛋鸡骨质疏松症，并设立对照组(钙含量 3. 7%)，分光光度法检测血清中钙、磷、碱性磷酸酶和抗酒
石酸酸性磷酸酶的含量;称量蛋鸡股骨和胫骨的重量;游标卡尺测量骨骼的长度、宽度;万能材料试验机进行三点弯曲实验检
测骨骼的强度;并制作观察骨组织切片进行 HE 染色，分析骨切片上成骨细胞、破骨细胞和骨细胞的变化。结果 研究发现与
对照组相比，低钙日粮导致了血清中碱性磷酸酶含量显著性增加，血清钙含量显著性下降，蛋鸡股骨和胫骨重量显著性变轻，
宽度显著性变窄，骨强度显著性降低。病理切片结果显示，与对照组相比，低钙组蛋鸡股骨远端骨切片中成骨细胞和破骨细
胞含量均增加，但骨细胞含量减少。结论 连续 60d 给蛋鸡饲喂低钙日粮可成功建立蛋鸡骨质疏松模型，且该模型为高转化
型骨质疏松。 |
| 英文摘要: |
| Abstract: Objective Cage layer osteoporosis is a common bone disease，which leads to a decline of eggshell quantity，an
increase of fracture rate，paralysis，and even death in hens． The disease has a big negative effect on the modern chicken industry．
The chicken osteoporosis model not only plays an important role in research of cage layer osteoporosis，but also in the human
osteoporosis． Methods In order to establish the model，the low calcium diet which only included 1. 5% of calcium was used for
inducing the cage layer osteoporosis． 3. 7% of calcium was used as control． The serum levels of calcium，phosphates，alkaline
phosphates (ALP)，and tartrate resistant acid phosphates (TＲACP) were detected using spectrophotometric method． The weight，
width，and length of the tibia and femur were measured． The strength of the tibia and femur were measured by 3-point bending test
using an universal material testing machine． Bone slices were HE-stained for the analysis of osteoblasts，osteoclasts，and osteocytes
on the slices． Ｒesults The experiment results showed that compared with those in the control group，the hens in low calcium
group had an increase of serum ALP and a decrease of calcium． The weight of the tibia and femur in low calcium group was lighter
than that in control group． The width of the tibia and femur in low calcium diet group was narrower． And the strength of the bone
was weaker． Pathological section of the distal femur results showed that the bone slices of the hens in low calcium group had more
osteoclasts and osteoblasts but less bone cells than in the control group． Conclusion The experiment suggests that the chicken
osteoporosis model can be induced by low calcium diet intake for 60 days． The model is a high bone-turnover model． |
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