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| 补肾、活血复方对骨质疏松症模型大鼠 VEGF 表达的影响 |
| Effect of the nourishing kidney and promoting blood circulation compound on VEGF expression in rat osteoporosis model |
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| DOI： |
| 中文关键词: 中医中药 补肾 活血 骨质疏松症 VEGF |
| 英文关键词:Chinese Medicine Nourishing kidney Promoting blood circulation Osteoporosis VEGF |
| 基金项目: |
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| 中文摘要: |
| 目的 比较补肾、活血复方对骨质疏松症模型大鼠 VEGF 表达的影响，探讨补肾、活血复方治疗骨质疏松症的分子机制。方法 以去卵巢骨质疏松症模型大鼠为实验研究对象，不同实验药物灌胃 12 周后，双能 X 线法测定骨密度，ELISA 法测定骨、肾及血清中 VEGF 含量，定量 PCＲ 法测定骨、肾中 VEGF mＲNA 相对表达量。结果 骨质疏松症模型大鼠骨密度、VEGF蛋白及 mＲNA 表达量显著降低，运用补肾、活血中药复方干预后，各组大鼠骨密度、VEGF 蛋白含量及 mＲNA 表达量显著升高。结论 肾虚血瘀骨代谢失常与 VEGF 表达降低有关，补肾、活血复方通过调节 VEGF 表达水平，起到治疗骨质疏松症的作用。 |
| 英文摘要: |
| Objective To compare the effect of the nourishing kidney and promoting blood circulation compound on VEGF
expression of rat osteoporosis model，and to investigate the molecular mechanism of the nourishing kidney and promoting blood
circulation compound in the treatment of osteoporosis． Methods Ovariectomized osteoporosis rat model was used for experimental
objective． The rats were given the medicines by intragastric administration for 12 weeks． Bone mineral density was measured using
dual-energy X-ray absorptiometry． The content of VEGF in the bone，kidney，and the serum were determined using ELISA method．
The relative expression of VEGF mＲNAs in the bone and the kidney were determined using qPCＲ method． Ｒesults The
expressions of VEGF protein and mＲNA in osteoporosis model rats decreased significantly． After the intervention with the
nourishing kidney and promoting blood circulation compound，the expressions of VEGF protein and mＲNA of each group increased
significantly． Conclusion The disorder of bone metabolism due to renal deficiency and blood stasis is related to low expression of
VEGF． The nourishing kidney and promoting blood circulation compound can treat osteoporosis through regulation of the expression
of VEGF． |
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