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| 唑来膦酸对人羊膜间充质干细胞移植治疗去卵巢大鼠骨质疏松协同效应研究 |
| Synergistic effect of zoledronic acid on human amniotic mesenchymal stem cell transplantation for treatment osteoporosis in ovariectomized rats |
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| DOI： |
| 中文关键词: 人羊膜间充质干细胞(AM-MSCs) 唑来膦酸 移植 去卵巢 大鼠 骨质疏松 |
| 英文关键词:AM-MSCs Zoledronic acid Transplantation Ovariectomized Ｒats Osteoporosis |
| 基金项目: |
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| 摘要点击次数: 977 |
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| 中文摘要: |
| 目的 探索唑来膦酸联合人羊膜间充质干细胞移植对去卵巢骨质疏松协同效应研究。方法 选择雌性未孕的 Wistar大鼠 45 只，完全随机分组分为假手术组、模型组及实验组，每组各 15 只。术后 第 7 天，行唑来膦酸灌胃 + 尾静脉注射 AM-MSCs 液(假手术组除外)，连续干预13 周，于末次干预后 24 h，处死大鼠并采集标本。每周测定1 次各组大鼠的体质量;双能X 线吸收骨密度仪分别测定左侧股骨中点、远端和近端骨密度;用原子吸收法测定其骨钙含量;干预 13 周后 ELISA 法检测血清 25-羟基维生素 D(25- HVD)、I 型胶原 C 端肽(CTX- I)、骨特异性碱性磷酸酶(BAP)、抗酒石酸酸性磷酸酶 5b
(TＲAP5b)的表达变化;检测各组大鼠生物力学性能的变化;ＲT-PCＲ 技术检测椎骨中转化生长因子 β1(TGF-β1)的基因表达。结果 假手术组大鼠体重逐渐增加，符合动物自然生长规律，模型组于造模后两个月开始，体重却明显高于假手术组，实验组体重渐增，与假手术组相近。与假手术组比较模型组的骨密度和骨钙含量均降低，与模型组比较，实验组的骨密度和骨钙含量含量均升高。与假手术组比较，模型组血清 25-HVD、CTX-I、BAP、TＲAP5b 的表达显著降低，与模型组比较，实验组显著升高。生物法力学检测显示，假手术组的极限载荷、极限应力和弹性模量比模型组有显著增高(P ＜ 0. 05)，实验组的极限载
荷和弹性模量比假手术组有显著增高(P ＜ 0. 05)。与假手术组、模型组比较，实验组 TGF-β1m ＲNA 的表达明显上调(P ＜0. 05)。结论 唑来膦酸协同人羊膜间充质干细胞移植具有较好改善去卵巢大鼠骨质疏松症的作用。 |
| 英文摘要: |
| Objective To investigate the synergistic effect of zoledronic acid on human amniotic mesenchymal stem cell transplantation for the treatment of osteoporosis in ovariectomized rats． Methods Forty-five female non-pregnant Wistar rats were randomly divided into sham operation group，model group，and experimental group，with 15 rats in each group． Stomach irrigation
of zoledronic acid and tail-vein injection of AM-MSCs (except in sham operated group) were performed after 7-day of the operation，and continued for 13 weeks． Twenty-four hours after the last intervention，the rats were killed and specimens were collected． The rat body weight was measured weekly． Bone mineral density (BMD) of the middle，distal，and proximal left femur was measured using dual energy X-ray absorptiometry． Calcium content of the bone was determined with atomic absorption method．Published online www． wanfangdate． com． cn doi:10. 3969/j． issn． 1006-7108. 2016. 09. 015
Changes of serum 25 HVD，CTX I，BAP，and TＲAP5b were detected using ELISA method after intervention for 13 week． The biomechanical properties of rats were detected． ＲT-PCＲ was used to detect TGF-β1 gene expression in the vertebrae． Ｒesults The weight of rats in the sham operation group increased gradually，in line with the natural growth． The weight was significantly
higher in model group than that in the sham operation group in 2 months after modeling． The weight increase in the experiment group was similar with the sham operation group． BMD and calcium content were lower in model group than in sham group． BMD and calcium content were higher in the experimental group than in the model group． Serum 25 HVD，CTX I，BAP，and TＲAP5b significantly decreased in model group comparing with those in the sham operation group． They increased significantly in the experimental group comparing with those in the model group． Mechanical biological detection showed that the limit load，limit stress，and elastic modulus increased significantly in sham operation group than in the model group (P ＜0. 05)． The limit load and
elastic modulus in the experimental group significantly increased than in the sham operation group (P ＜ 0. 05)． TGF-β1 mＲNA expression significantly increased in experimental group than in the sham operation group and model group (P ＜0. 05)． Conclusion Zoledronate phosphoric acid has beneficial effect in ovariectomized rats with osteoporosis when works together with amniotic mesenchymal stem cell transplantation． |
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