|
| 垂直振动对大鼠腰椎小关节退变及骨密度影响的实验研究 |
| An experimental study on the effects vertical vibration on lumbar facet joint degeneration and bone mineral density in rats |
| |
| DOI：10.3969/j.issn.1006-7108.2016.10.005 |
| 中文关键词: 振动；腰椎小关节；免疫组化 原位末端标记法 |
| 英文关键词:Vibration Lumbar facet joint Immunocytochemistry TUNEL |
| 基金项目:全军后勤“十二五”科研课题(CWS11J170) |
|
| 摘要点击次数: 1394 |
| 全文下载次数: 792 |
| 中文摘要: |
| 目的 观察大鼠腰椎小关节突在不同振动频率下关节软骨退变程度及腰痛相关因子的表达情况，同时探究振动对骨密度的影响。方法 收集对照组和振动组（振动组接受15min/d，频率5~60Hz、振幅3mm的垂直振动12w）大鼠腰椎小关节软骨，采用HE染色和AB-PAS染色，进行Mankin评分评价软骨退变程度；免疫组化染色观察凋亡软骨细胞、COX-2、PGE2、CII在小关节突软骨表达；双能X线吸收法测量振动前后股骨颈骨密度。结果 对照组、5Hz振动组、20Hz振动组、60Hz振动组小关节Mankin评分依次增加，有显著性差异(P<0.05)；组间凋亡软骨细胞、COX-2、PGE2表达随着振动频率增加而显著性增强(P<0.05)；对照组与5Hz振动组CII表达差异不明显（P>0.05），其余各组间CII表达差异明显(P<0.05)；组间骨密度增加值无显著性差异。结论 长时间垂直振动可导致软骨退变，振动频率越大，小关节退变越重；振动与致痛因子表达具有相关性；5~60Hz、3mm振幅振动不能促进幼龄大鼠BMD增加。 |
| 英文摘要: |
| Objective To investigate the effects of different vibration frequencies on the occurrence of rat lumbar facet joint degeneration, low back pain-related factors and femur bone mineral density in rats. Methods The lumber facet joints were harvested from rats in control group and vibration group (Vibration group rats were applied 15min/d, 5-60Hz frequency, amplitude of 3mm vertical vibration for 12 weeks). Sections of facet joints were stained with HE staining and AB-PAS staining, and the severity of cartilage degeneration was determined according to the Mankin score. Immunohistochemistry was used to examine the expression of apoptotic cells, CII COX-2 and PGE2. DXA was used to measure bone mineral density of femoral neck before and after the vibration. Results With the increase in Herz, the Mankin scores increased, and the severity of facet joint degeneration was significantly different among the groups (P<0.05). The number of apoptotic cells and content of COX-2 and PGE2 increased significantly with increased frequency (P<0.05). The expression difference of CII between control group and 5Hz group was not statistically significant, but the difference among other groups was significant. The BMD of different groups was not significantly different. Conclusions Long-term vertical vibration can lead to cartilage degeneration, the higher the frequency, the greater the small joint degeneration. Vertical vibration was related to the expression of pain-related factors. 5-60Hz, 3mm amplitude of vertical vibration could not increase BMD of femoral neck in young-age rats. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |