| Objective The present study was designed to evaluate the effects of Zuogui Wan and Yougui Wan and their disassembled prescriptions on PPARγ, LPL and FABP4 mRNA levels of bone marrow mesenchymal stem cells in ovariectomized rat. Method Serum of control Group (CON), ovariectomized group (OVX), sham group (SHAM), Zuogui Wan group (ZGW), Yougui Wan group (YGW), common prescription group (GTY), Yin prescription group (ZYY), Yang prescription group (BYY), progynova group (BJL) were prepared, and they simultaneously intervened the bone marrow mesenchymal stem cells (BMSCs). BMSCs proliferation was tested by the method of MTT, and BMSCs were identified by FCM (flow cytometry). The mRNA expression of Peroxisome Proliferator-Activated Receptor γ (PPARγ), lipoprotein (LPL) and fatty acid binding protein 4 (FABP4) were assayed using Q-PCR. Result All the serum containing Zuogui Wan and Yougui Wan and their disassembled prescriptions downregulated PPARγ, LPL and FABP4 mRNA. Especially, Yougui Wan remarkably downregulated PPARγ, LPL and FABP4 mRNA (P<0.05). Conclusion Zuogui Wan and Yougui Wan and their disassembled prescriptions can inhibit adipogenic differentiation .Yuogui Wan may have a higher level of inhibition on adipogenic differentiation than Zuogui Wan, yang prescription and common prescription. |