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| 血清COL10A1在骨关节炎中检测的意义 |
| Detection of serum COLl0Al level in patients with knee osteoarthritis |
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| DOI：10.3969/j.issn.1006.7108.2016.11.002 |
| 中文关键词: X型胶原 骨性关节炎 COLl0Al |
| 英文关键词:Type X collagen Osteoarthritis COLlOAl |
| 基金项目:国家自然科学基金项目资助（U1503221) |
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| 中文摘要: |
| 目的 观察并探讨血清COLl0Al水平在骨关节炎软骨损伤的诊断价值以及对骨性关节炎疾病活动性严重程度的预测价值。方法 所收集89例KOA患者均为2014年9月至2016年4月在我院关节外科门诊就诊和住院患者，采集清晨空腹血 5ml,依据影像学Kellgren-Lawrenc(K-L)分级评分系统进行病情严重程度分级，I级20例，Ⅱ级25例，Ⅲ级23例，IV级21例，正常对照组23例。各组年龄及性别构成比无显著性统计学差异，所有血清标本应用双抗体夹心酶联免疫吸附（ELISA)法检 测COLl0Al浓度。结果 1、KOA组患者血清COL10A1(1. 19(0.61 - 1. 93 ) ng/ml)浓度水平较正常对照组（0. 45 (0. 34 -0.52)ng/ml)高，且差异具有显著性统计学意义（P <0.01)。2、在骨关节炎病情预测上，血清COLl0Al浓度水平在OA患者 K-L分级II级组（1.98(1. 59 -2. 12)ng/ml)和Ⅲ级组（1. 84( 1. 43 -2. 02)ng/ml)最高，且与I级组和IV级组差异具有显著统计学意义（P <0.01)，其中Ⅱ级组与Ⅲ级组患者血清COL10A1浓度水平差异不具有统计学意义（P =0.149)，二者无显著差异。结论 1、骨性关节炎患者血清COLl0Al浓度水平较正常对照组高。2、在骨关节炎组,血清COLl0Al水平随着K-L分级等级逐渐升高，在Ⅱ级组和Ⅲ级组时达到最高，随后逐渐下降，因此具有结合影像学K-L分级预测评价患者病情严重程度的潜力。 |
| 英文摘要: |
| Objective To observe the relationship between the serum COLl0Al level and the severity of knee osteoarthritis (KOA). Methods Eighty-nine patients with KOA in our outpatient and inpatient department from September 2014 to April 2016 were selected. Five ml morning serum of each subject was collected and lateral position of X-ray detection of knee joint was performed. The X-ray images were classified according to the Kellgren-Lawrence radiographic grading criteria. All the subjects were divided into group I (n = 20), group II (n = 25)，group III (n = 23)，group IV (n =21), and control group (n = 23). There were no significant differences of age and gender among the groups. The serum COLl0Al level was detected using enzyme-linked immunosorbent assay (ELISA). Results 1) The serum COLl0Al level in KOA group (1. 19 ng/ml, 0. 61 -1.93 ng/ml) was significantly higher than that in control group (0.45 ng/ml, 0.34 - 0.52 ng/ml, P < 0. 01). 2) In group KOA, the serum COLl0Al levels in group II (1. 98 ng/ml, 1. 59 -2. 12 ng/ml) and group III (1. 84 ng/ml, 1. 43 -2. 02 ng/ml) were the highest, and no significant difference of the serum COLl0Al level between group II and group III was observed (P >0. 05). Among them, it was significantly different between group I and IV (P < 0. 01). Conclusions 1) The serum COLl0Al level in patients with KOA is higher than that in normal subjects. 2) In KOA group, the serum COLl0Al level increases gradually, and reaches to the highest in group II and group III, and then decreases gradually. So it has potential to evaluate the severity of the patient’s condition combining with the K-L classification. |
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