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| 补肾健脾中药复方干预成骨细胞内细胞凋亡蛋白的机制研究 |
| Study of the mechanism of Chinese herbal compound of tonifying kidney and strengthening spleen to interfere the apoptotic proteins in osteoblasts |
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| DOI：10.3969/j.issn.1006.7108.2016.11.006 |
| 中文关键词: 中医中药 成骨细胞 补肾健脾 凋亡蛋白 机理研究 |
| 英文关键词:Chinese medicine Osteoblast Tonifying kidney and strengthening spleen Apoptotic protein Mechanism study |
| 基金项目:国家自然科学基金项目（81001532)；中国博士后基金（20100480737);广东省自然科学基金项目（2014A030313748)；广东省科技计划项目（2013B021800162;2010B030700076);广州市珠江科技新星项目（2012J2200037） ;佛山市科技创新平台建设项目（2013AG100022) |
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| 中文摘要: |
| 目的 分析补肾健脾中药复方对成骨细胞内Bcl-2、Bax和Bid的调控作用，探讨细胞凋亡信号途径和骨质疏松症的相关关系。方法 选用1日龄SD大鼠获取原代的成骨细胞体外培养，细胞分成治疗组、对照组和正常组，治疗组给予补肾健脾中药复方含药血浆，对照组给予戊酸雌二醇含药血浆培养，正常组常规培养，采用MTT比色法记录各组细胞的OD值,荧光定量PCR法检测各组Bcl-2、Bax和Bid表达。结果 在细胞增殖方面，治疗组的OD值在第8天时达最大值，明显高于对照组 (P <0.05),从而确定第8天为最佳实验时间；与对照组相比，治疗组中Bcl-2、Bax和Bid蛋白的表达量上升，其差异有统计学 意义（P<0.05);而且治疗组的Bcl-2/Bax比值明显高于对照组,其差异有统计学意义（P <0.05)。结论 补肾健脾中药复方能下调Bcl-2、Bax和Bid的表达，在一定程度上抑制成骨细胞的凋亡。 |
| 英文摘要: |
| Objective To analyze the intervention effect of Chinese herbal compound of tonifying kidney and strengthening spleen on Bcl-2, Bax, and Bid in osteoblasts, and to explore the relationship between the apoptosis signal pathway and osteoporosis. Methods Osteoblasts were harvested from 1 -day-old SD and cultured. Cells were divided into observation group, control group, and normal group. Cells in observation group were cultured with medium of drug containing plasma of Chinese herbal compound. Cells in control group were cultured with medium of drug containing plasma of estradiol valerate. Cells in normal group were given routine culture. Cell OD value in each group was recorded using MTT assay. Expression of Bcl-2, Bax, and Bid protein in each group was detected with fluorescence quantitative PCR. Results In cell proliferation, the OD value in observation group reached the maximum in 8 days, which was significantly higher than that in control group (P<0. 05) . So it was determined that the 8th day was the best time for the experiment. The expression of Bcl-2, Bax, and Bid protein in observation group increased significantly in observation group compared to that in control group, and the difference was significant (P <0, 05). Bcl-2/Bax ratio in observation group was higher than that in control group, and the difference reached statistical significance (P < 0. 05). Conclusion Chinese herbal compound of tonifying kidney and strengthening spleen down regulates the expression of Bcl-2, Bax, and Bid protein, and inhibits the apoptosis of osteoblasts to a certain extent. |
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