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| 强骨饮对去卵巢大鼠骨折愈合骨小梁Micro-CT改变研究 |
| The effect of strengthening-bone receipt on the change of trabecular bone of fracture healing under micro-CT in rat osteoporotic model |
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| DOI：10.3969/j.issn.1006.7108.2016.11.007 |
| 中文关键词: 中医中药 骨质疏松 强骨饮 骨折 Micro-CT 动物实验 |
| 英文关键词:Chinese medicine Osteoporosis The strengthening-bone receipt Fracture Micro-CT Animal experiment |
| 基金项目:浙江省自然科学基金（Y14H270017) |
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| 中文摘要: |
| 目的 研究强骨饮对去卵巢大鼠骨折愈合骨小梁Micro-CT的影响。方法 将32只雌性SD大鼠随机分为4组：A组 (假手术组）、B组（模型组）、C组（强骨饮组）、D组（基础用药组）。骨松造模组（B、C、D组）术前随机取8只大鼠进行骨密度测量，后骨松造模组摘除双侧卵巢，A组切除少许脂肪后缝合，3月后随机取骨松造模组8只大鼠进行骨密度測量以证明造模成功;将所有大鼠进行骨折造模，后将C组强骨饮灌胃、D组进行碳酸钙和维生素D3灌胃，A、B两组用等量生理盐水灌胃；在 6周后时各取8只大鼠股骨骨折处骨痂进行Micro-CT分析，参数包括BV/TV、Tb. Th、Tb. N、Tb. Sp。结果 B组骨小梁数量、骨小梁厚度与骨体积分数低于C、D组（P <0.05),骨小梁分离度指数高于C、D组（P<0. 05) ；C组与D组骨小梁数量、骨小梁厚度比较差异有统计学意义（P <0.05)，而与D组的骨小梁分离度及骨体积分数比较差异无统计学意义（P >0.05)。结论 强骨饮能增加骨量、改善骨质疏松骨三维结构特别是增加骨小梁数量与厚度，其可能机制是强骨饮通过抑制破骨细胞活性，从而增强骨强度、促进骨折愈合。 |
| 英文摘要: |
| Objective To study the effect of the strengthening-bone receipt on the trabecular bone in an osteoporotic rat model of fracture healing with micro-CT. Methods 32 female rats were randomly divided into 4 groups, group A (sham group)，group B (OVX group), group C (strengthening-bone receipt group), and group D (basic treatment group). BMD was detected in 8 rats of model groups (B，C, and D group) before surgery. The rats in model groups were then ovariectomized. Rats in group A were excised small amount of fat and sewn. Eight rats were randomly selected for BMD measurement to prove the successful model establishment in 3 months. Fracture model was conducted in all rats. Rats in group C received the strengthening-bone receipt with gavage. Rats in group D received calcium and vitamin D with gavage. Rats in group A and B received normal saline. Rats were killed in 6 weeks. The femur fracture callus was collected and the parameters including bone volume fraction (BV/TV), trabecular thickness (Tb. Th), trabecular number (Tb. N), and trabecular separation (Tb. Sp) were analyzed using micro-CT. Results The Tb. N, Tb. Th, and BV/TV in group B were less than those in group C and D (P <0. 05) , but the Tb. Sp was higher than that in group C and D (P < 0. 05).The Tb. N and Tb. Th were significantly different between group C and group D (P <0. 05), while the Tb. Sp and BV/TV between group C and group D were not significantly different (P >0. 05). Conclusion The strengthening- bone receipt increases bone mass and improves three-dimensional structure of the bone, particularly Tb. N and Tb. Th. The possible mechanism of enhancing bone strength and promoting fracture healing may be through inhibiting the activity of osteoclasts. |
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