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| miR-137靶向作用RUNX2调控MC3T3-E1细胞成骨分化 |
| Targeting of RUNX2 by miR-137 and its regulation on MC3T3-E1 cells osteogenic differentiation |
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| DOI：10.3969/j.issn.1006.7108.2016.11.016 |
| 中文关键词: miR-137 RUNX2 MC3T3-El细胞 成骨分化 |
| 英文关键词:miR-137 RUNX2 MC3T3-E1 cell Osteogenic differentiation |
| 基金项目:广东省医学科研基金项目（B2014313)；广东省创新强校工程青年创新人才类项目（2014KQNCX093)；湛江市科技计划项目（2014A06005 ) ；广东医学院科研基金面上培育项目 (M2015013) |
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| 中文摘要: |
| 目的 探讨miR-137对RU1VX2的靶向作用及其对MC3T3-E1细胞成骨分化的影响。方法通过软件预测miR-137与成骨标志基因RUNX2的结合位点。检测MC3T3-E1细胞诱导成骨分化过程中miR-137的表达变化。转染miR-137 mimics或 miR-137 inhibitor后,再诱导MC3T3-E1细胞成骨分化,检测成骨分化标志物（RUNX2、ALP、OPN、OCN及OSX)表达变化。结 果 经软件预测，miR-137与靶基因RUNX2有结合位点。MC3T3-E1细胞成骨分化过程中，miR-137表达下调。转染miR-137 mimics的MC3T3-E1细胞成骨标志基因表达受到抑制，而转染miR-137 inhibitor组成骨标志基因呈不同程度表达上调。结论 miR-137通过靶向作用RUNX2基因调控MC3T3-E1细胞成骨分化。 |
| 英文摘要: |
| Objective To investigate the targeting of RUNX2 by miR-137 and its effect on MC3T3-E1 cell differentiation. Methods The binding site of miR-137 and osteogenic marker gene RUNX2 was predicted by using an online prediction program. The expression level of miR-137 in MC3T3-E1 cells was detected during osteogenic differentiation. After transfection with miR-137 or miR-137 inhibitor, the MC3T3-E1 cells were induced to differentiate into osteoblasts, and the expression of osteogenic differentiation markers (RUNX2, ALP, OPN，OCN and OSX) were detected. Results The RUNX2 gene as a potential target of miR-137 was identified using the miRNA target prediction program. miR-137 expression is down regulated during osteogenic differentiation of MC3T3-E1 cells. The expression level of osteogenic marker genes was inhibited in MC3T3-E1 cells after transfected with miR-137. However, the expression level of osteogenic marker genes was up-regulated after transfected with miR- 137 inhibitor. Conclusion miR-137 regulates the osteogenic differentiation of MC3T3-E1 cells by targeting the RUNX2 gene. |
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