|
| 人胎盘提取物对成骨细胞增殖、分化、矿化及骨愈合的实验研究 |
| Effect of human placental extract on proliferation, differentiation and mineralization of osteoblasts |
| |
| DOI：10.3969/j.issn.1006.7108.2016.12.001 |
| 中文关键词: 人胎盘提取物 成骨细胞 骨愈合 矿化 |
| 英文关键词:Human placenta extract Osteoblast Fracture healing Mineralization |
| 基金项目:国家自然科学基金项目（81560266)；贵州医科大学附属医院博士启动基金 |
|
| 摘要点击次数: 707 |
| 全文下载次数: 573 |
| 中文摘要: |
| 目的 研究人胎盘提取物（human placenta extract，HPE )对体外成骨细胞的增殖、分化、矿化的影响，进一步了解HPE促进骨折愈合的机理。方法 用成骨细胞系（hFobl. 19细胞），通过CCK-8、流式细胞术、Real-Time PCR法分别检测不同剂量的 HPE(0、18μL、100μL、180μL、360μL)在不同时间对成骨细胞增殖的影响；通过检测碱性磷酸酶（alkaline phosphatase, ALP)观察不同剂量的 HPE 和 Rhbmp-2(0、18μL HPE ,100μL HPE,100μL Rhbmp-2 、100μL HPE + 100μL Rhbmp-2)在不同时间对成骨细胞分化的影响；通过茜素红染色检测不同剂量的HPE(0、50μL、100μL、200μL)在不同时间对成骨细胞矿化的影响，系统研究体外HPE对成骨细胞增殖、分化、矿化的影响及其促进骨愈合的可能机制。结果 空白对照组和加入不同剂量的HPE各组对成骨细胞的增殖、分化未观察到明显的影响（P>0. 05)，而加人不同剂量的HPE(50μL、100μL、200μL)各组对成骨细胞的矿化比空白对照组有明显促进作用（P<0.01)。结论 体外HPE对成骨细胞的增殖、分化无明显影响，对成骨细胞的矿化有明显促进作用；HPE促进成骨细胞矿化可能是促进骨折愈合机制。 |
| 英文摘要: |
| Objective To evaluate the effect of human placenta extract (HPE) on the proliferation, differentiation and mineralization of primary osteoblasts, and to further explore the possible mechanism for improving fracture healing. Methods Osteoblast cell line, hFobl. 19，was used for the overall study. Osteoblasts were treated with HPE of different doses (0，18μL, 100μL, 180μL, 360μL)，and the cell proliferation was determined by CCK-8, FACS or Real-Time PCR. Alkaline Phosphatase production was assessed to evaluate the cell differentiation level. The effect of HPE (0，50μL，100μL, 200μL) on the mineralization of osteoblasts was detected by Alizarin Red S Staining. Results Compared with the control group, the mineralization of osteoblast increased after treatment with higher doses of HPE (P < 0. 01)，but no significant enhancement of cell proliferation and cell differentiation were observed after cultured with different doses of HPE (P > 0. 05). Conclusion Our study suggests that HPE may contribute to fracture healing not through improvement of osteoblast proliferation or differentiation, but through enhancement of bone mineralization. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |