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| 髋骨性关节炎滑膜组织中钙结合蛋白（S100A12）的表达及意义 |
| The expression and significance of calcium binding protein (S100A12) in synovial tissues of hip osteoarthritis |
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| DOI：10.3969/j.issn.1006-7108.2017.01.006 |
| 中文关键词: S100A12 髋骨性关节炎 滑膜 |
| 英文关键词:S100A12 Hip osteoarthritis Synovial |
| 基金项目:南京市卫生局医学科技发展项目(YKK13218) |
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| 中文摘要: |
| 目的 观察髋骨性关节炎患者血液及滑膜组织中钙结合蛋白S100A12的表达，以探讨它在髋骨性关节炎致病过程中的作用，为揭示髋关节骨性关节炎发病机制提供理论和实验依据。 方法 收集行髋关节置换的27例髋骨性关节炎患者（OA组）与35例股骨颈骨折患者（对照组）；对OA组患者的髋关节X片分析，根据Kellgren-Lawrence（K-L）分级标准对患者进行分级；对两组患者静脉采血，术中取滑膜组织，采用ELISA法定量和免疫组化技术检测S100A12的表达，并采用IPP软件半定量分析免疫组化S100A12的表达量。结果 OA组的血清中S100A12水平（94.20±49.85）ng/mL显著高于对照组含量（25.32±16.87）
ng/mL，有统计学差异（P<0.01）；滑膜组织OA组与对照组S100A12含量分别为(3.73±1.97)ng/mg总蛋白和(1.67±1.21)ng/mg总蛋白（P<0.01），差异有统计学意义；免疫组化分析
S100A12阳性反应在滑膜微血管内中性粒细胞胞浆中，以及部分滑膜细胞的胞浆内。OA组平均光密度MOD（0.21±0.06）较对照组MOD（0.13±0.05）有显著性差异（P<0.01）；OA组K-L分级4级患者的血清中S100A12水平（104.81±46.68）ng/mL显著高于3级血清含量（86.85±52.46）ng/mL，有统计学差异（P<0.01）。4级患者滑膜组织含量(4.62±1.95 )ng/mg总蛋白显著高于3级患者水平(3.13±1.82)ng/mg总蛋白（P<0.01）。结论 S100A12在髋骨关节炎患者的滑膜组织表达量显著增加； S100A12的表达与患者髋骨关节炎严重程度相关；S100A12的检测有助于骨性关节炎的诊断，为临床治疗提供参考。 |
| 英文摘要: |
| Objective To investigate the expression of calcium binding protein S100A12 in synovial tissue and serum of hip osteoarthritis, to study its role in the hip osteoarthritis pathogenesis, and to provide theoretical and experimental evidence. Methods Twenty-seven hip osteoarthritis patients for hip replacement (OA group) and 35 cases of femoral neck fracture patients (control group) were studied. The progression of osteoarthritis was classified with Kellgren- Lawrence grading scale by evaluating X-ray images of the hip joint. Serum samples and synovial tissue from two groups were collected before and during operation. S100A12 content and expression were analyzed using enzyme-linked immunosorbent assay (ELISA) and immunohistochemical (IHC) staining. Results Serum S100A12 levels in OA group (94.20±49.85) ng/ml were higher than that in control group (25.32±16.87) ng/mL (P<0.01). The S100A12 content in synovial tissue of OA group (3.73±1.97 ng/mg total protein) was higher than that in controls (1.67.±1.21 ng/mg total protein, P<0.01). IHC analysis showed that S100A12 was positive in the cytoplasm of neutrophils in the synovial capillaries, as well as in the cytoplasm of some synovial cells. The mean optic density (MOD) in OA group (0.21±0.06) was significantly higher than that in control group (0.13±0.05, P<0.01). The level of S100A12 in serum of grade 4 by K-L grading scales (104.81 + 46.68 ng/ml) was significantly higher than that in the level of grade 3 (86.85 + 52.46 ng/mL, P<0.01). The level of S100A12 in synovial tissue of grade 4 (4.62 + 1.95 ng/mg total protein) was significantly higher than that in the level of grade 3 (3.13 + 1.82 ng/mg total protein, P<0.01). Conclusion The expression of S100A12 significantly increases in synovial tissue of patients with hip osteoarthritis. The levels of S100A12 are closely related to the severity of hip osteoarthritis, which can be used as an effective biochemical marker for early detection of osteoarthritis and the evaluation of clinical therapeutic of OA. |
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