|
| 1,25(OH)2D3对人骨肉瘤MG63细胞基质GLA蛋白及Wnt信号通路的影响 |
| The effect of 1,25-dihydroxyvitamin D3 on matrix Gla protein and Wnt/β-catenin signaling pathways in human osteosarcoma MG63 cells line |
| |
| DOI：10.3969/j.issn.1006-7108.2017.01.008 |
| 中文关键词: 维生素D 基质Gla蛋白 骨肉瘤细胞 Wnt/β-catenin信号通路 骨质疏松 |
| 英文关键词:Vitamin D Matrix gla protein Human osteosarcoma cells Wnt/β-catenin signaling pathway Osteoporosis |
| 基金项目:国家自然科学基金（81460171);江西省自然科学基金(20142BAB205016) |
|
| 摘要点击次数: 1151 |
| 全文下载次数: 794 |
| 中文摘要: |
| 目的 观察1,25(OH)2D3对人成骨肉瘤MG63细胞株Wnt信号通路及MGP表达的影响，探讨其在骨质疏松发病中可 能的新机制。方法 分别使用 10–8 mol/L 1,25(0H)2D3、200 ng/mL Wnt 信号通路阻断剂 DKK-1、10–8 mol/L l,25(OH)2D3 + 200 ng/mLDKK-1干预人骨肉瘤细胞MG63细胞株48 h，使用实时荧光定量RT-PCR及Western-blot技术测定Wnt/β-catenin信 号通路中相关因子β-catenin、Runx2、LRP5及MGP基因及蛋白表达的影响。结果（1)10–8 mol/L 1,25(0H)2D3上调MG63 细胞中Wnt/β-catenin信号通路中相关因子β-catenin、Runx2、LRP5及MGP的基因表达及蛋白的表达，其中上调基因分别是 对照组的2.73倍、3.72倍、1.53倍、2.31倍，差异有统计学意义（P <0. 05);上调蛋白表达分别是对照组的1.13倍、1.17倍、 1.14倍、1.21倍，差异有统计学意义（P <0.05)。(2)DKK1下调β-catenin、LRP5、Runx2、MGP的基因及蛋白表达，其中下调基因表达分别是对照组的0.34倍、0.52倍、0.42倍、0.78倍，差异有统计学意义（P < 0.05)。下调蛋白表达分别是对照组的0.93倍、0.92倍、0.87倍、0.86倍，差异均有统计学意义（P<0.05)。结论1,25(OH)2D3有可能通过Wnt/β-catenin信号通路影响MGP的表达。 |
| 英文摘要: |
| Objective To observe the effects of 1,25-dihydroxyvitamin D3 on Wnt/β-catenin signaling pathways and on the expression of MGP in human osteosarcoma cells line (MG63) in vitro， and to investigate the potential role of vitamin D in postmenopausal osteoporosis. Methods Human osteosarcoma MG63 cells were treated with the following drug intervention respectively：10-8 mol/L 1,25(OH)2D3，200 ng/mL DKK-1，or 10-8 mol/L 1,25(OH)2D3+200 ng/mL DKK-1 for 48 hours，then total RNA and protein were extracted and examined by Real-Time quantitative RT-PCR and Western blot to measure the mRNA and protein expression of β-catenin，Runx2，LRP5，MGP in MG63 cells. Results 10-8 mol/L 1，25( OH )2D3 significantly up- regulated the expression of classic Wnt/β-catenin signaling pathway associated proteins such as β-catenin，Runx2，LRP5 and MGP. The mRNA expression increased by 2.73-fold，3.72-fold，1.53-fold and 2.31-fold，respectively，compared with the control group， and the protein expression increased by 1.13-fold，1.17-fold，1.14- fold and 1. 21-fold，respectively，compared with the control group，and the differences were statistically significant (P <0. 05). However，DKK1 down-regulated the expression of β-catenin, LRP5, Runx2 and MGP. The mRNA expression decreased by 0.34-fold，0.52-fold，0.42-fold and 0.78-fold，respectively，and the protein expression decreased by 0. 93-fold, 0.92-fold, 0.87-fold and 0.86-fold, respectively, compared with the control group and the differences were statistically significant (P <0.05). Conclusion The classic Wnt/β-catenin signaling pathway may play an important part in postmenopausal osteoporosis. 1,25 (OH) 2D3 may regulated the expression of MGP through the classic Wnt/β- catenin signaling pathway. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |