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| 联合使用固力康和特立帕肽对去卵巢大鼠股骨干骺端骨量、骨强度的影响 |
| The influence of combined treatment with Teriparatide and Menatetrenone on bone mass and strength of the metaphysis of femur in OVX rats |
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| DOI：10.3969/j.issn.1006.7108.2017.02.019 |
| 中文关键词: 去势大鼠 骨质疏松症 固力康 特立帕肽 |
| 英文关键词:OVX rats Osteoporosis Menatetrenone Teriparatide |
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| 中文摘要: |
| 目的 探索联合使用特立帕肽（PTH)和固力康（KKI)对去势大鼠骨质疏松的防治作用。方法 50只健康雌性SD大鼠随机行假手术（Sham，n = 10)和切除双侧卵巢（OVX,n =40)手术后，OVX大鼠随机的分成4组：OVX组、KKI组、PTH组、 PTH + KKI组。术后第一天开始给予药物治疗，KKI组：固力康灌胃（剂量6 mg/kg,每天1次），PTH组：PTH皮下注射（剂量 60 ng/kg,每周3次），PTH + KKI组：固力康灌胃和特立帕肽皮下注射，剂量和用药频率和KKI组、PTH组相同，直至术后12 w 为止，12 w时所有大鼠处死取股骨行Micro-CT、HE染色切片及骨生物力学检测。结果 结果表明KKI组、PTH组、PTH + KKI组和OVX组相比，股骨干骺端都有较高的BMD、BV/TV、Tb. Th、Tb.N、Corm. D、最大的载荷、刚度和较低的Tb. Sp,其中 PTH + KKI组大鼠胫骨有最高的BMD、BV/TV、Tb. Th、Tb. N、Corm. D、最大的载荷、刚度和最低Tb. Sp。KKI和PTH单独使用的效果明显低于他们联合使用对去卵巢大鼠骨质疏松的防治作用。结论 联合使用固力康和特立帕肽对去势大鼠骨质疏松的防治有叠加作用。 |
| 英文摘要: |
| Objective This study was designed to investigate the effect of combined treatment with Menatetrenone and Teriparatide on osteoporosis in OVX rats. Methods After bilateral ovariectomy (OVX, n = 40) or sham operation (Sham, n = 10) in healthy female SD rats, the rats in OVX group were randomly divided into 4 groups：group OVX, group KKI, group PTH, and group PTH + KKI. One-day after the operation, the rats in groups PTH, KKI, PTH + KKI received Teriparatide (60μg/kg, 3 times a week), or Menatetrenone soft capsules (6mg/kg/d), or Teriparatide (60μg/kg, 3 times a week) plus Menatetrenone soft capsules (6mg/kg/d)，respectively, for 12 weeks. The femurs of the rats were harvested and HE stained for micro-computerized tomography, histology, and biomechanical evaluation. Results Comparison with those in group OVX, the metaphysic of the femurs from groups KKI, PTH, and PTH + KKI had a higher BMD, BV/TV，Tb. Th, Tb. N, Conn. D, maximum load, and stiffness, but a lower Tb. Sp. The maximal BMD, BV/TV, Tb. Th, Tb. N，Conn. D, maximum load, and stiffness, and the minimal Tb. Sp were found in PTH + KKI group. The effect of Menatetrenone or Teriparatide use alone was significantly lower than their combined use for prevention and treatment of osteoporosis in OVX rats. Conclusion The application of Teriparatide plus Menatetrenone has an additive effect on osteoporosis in OVX rat. |
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