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| 葛根素联合国产多孔钽对人成骨细胞COL-I、OC、OPN表达和细胞增殖的影响 |
| The influence of puerarin combined with domestic porous tantalum on the expression of COL-I, OC, OPN and cell proliferation of osteoblasts |
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| DOI：10.3969/j.issn.1006.7108.2017.02.021 |
| 中文关键词: 中医中药 葛根素 多孔钽 I型胶原 骨钙素 骨桥蛋白 |
| 英文关键词:Chinese medicine Epuerarin Porous tantalum Type I collagen Osteocalcin Osteopontin |
| 基金项目:国家科技部科技支撑课题（2012BAE06B03) ;河北省科技支撑资助项目（16277776D);河北省医学科学研究重点课题计划资助项目（20160225);河北省卫生计生委临床医学优秀人才研究项目（361036);华北理工大学博士科研启动基金资助项目 |
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| 中文摘要: |
| 目的 研究探索葛根素联合国产多孔钽支架材料对人成骨细胞MG-63 COL-I、OC、OPN表达和细胞增殖的影响。方法 实验分为：葛根素组：不同浓度的葛根素与成骨细胞共培养（CCK-8法筛选出葛根素作用最佳剂量浓度组）；多孔钽组：国产多孔钽与成骨细胞共培养;葛根素多孔钽组：葛根素、国产多孔钽与成骨细胞共培养；空白组：成骨细胞培养。扫描电镜观察多孔钽及葛根素多孔钽-成骨细胞复合物表面形态；CCK-8法检测成骨细胞的生长增殖状态；实时荧光定量PCR法（RT-PCR)和免疫细胞化学染色法检测成骨细胞COL-I、OC、OPN mRNA和蛋白的表达。结果葛根素和多孔钽组较空白组均可促进成骨细胞的增殖（P<0.05);葛根素多孔钽组较其它各组细胞的增殖活性最显著（P <0.05)。RT-PCR法和免疫细胞化学染色法结果显示，葛根素和国产多孔钽对成骨细胞COL-I、OCmRNA和蛋白表达均有影响（P < 0. 05),但对OPN mRNA和蛋白无显著影响（P>0.05)。结论 葛根素和国产多孔钽支架具有协同影响成骨细胞COL-I、OC、OPN mRNA和蛋白的表达，以及促进成骨细胞的增殖。 |
| 英文摘要: |
| Objective To explore the influence of Puerarin combined with domestic porous tantalum on the expression of COL-I, OC and OPN and cell proliferation of osteoblasts. Methods There were four experimental groups including Puerarin group ： different concentrations Puerarin co-cultured with osteoblasts (CCK-8 method was used to screen the optimal concentration dose group) ； Porous tantalum group： domestic porous tantalum co-cultured with osteoblasts； Puerarin porous tantalum group： Puerarin and domestic porous tantalum co-cultured with osteoblasts; Blank group： osteoblast culture. Scanning electron microscopy was used to observe the surface morphology of porous tantalum and puerarin porous tantalum osteoblasts. CCK-8 method was used to detect the growth and proliferation of osteoblasts. Real-time fluorescence quantitative PCR assay (RT-PCR) and immunocytochemistry staining were used to detect the expression of COL-I, OC, mRNA OPN and protein in osteoblasts. Results Compared with the blank group，the proliferation of osteoblasts could be promoted by puerarin and porous tantalum ( P < 0. 05). Compared with other groups, the proliferation activity of the puerarin porous tantalum group was the most significant (P <0.05). RT-PCR and immunocytochemistry staining showed that Puerarin and domestic porous tantalum have effect on the expression of COL-I, OCmRNA and protein in osteoblasts (P < 0. 05 ) , but there was no signiticant effect on OPN mRNA and protein ( P > 0. 05 ). Conclusion Puerarin and domestic porous tantalum stent have synergistic effect the expression of COL-I，OC mRNA OPN and protein, as well as promoting the proliferation of osteoblasts. |
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