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| 大鼠卵巢去势后颌骨和股骨骨髓基质干细胞性能观察 |
| The comparison of the characteristics between bone marrow mesenchymal stem cells derived from the mandible and the femur in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2017.05.003 |
| 中文关键词: 骨髓基质干细胞 骨质疏松 卵巢切除 |
| 英文关键词:Mesenchymal stem cells Osteoporosis Ovariectomy |
| 基金项目:武警后勤科研项目(wjh20110256） |
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| 中文摘要: |
| 目的 观察去卵巢骨质疏松大鼠颌骨及股骨来源的骨髓间充质干细胞增殖及成骨分化能力的差异。 方法 雌性SD大鼠双侧卵巢切除建立骨质疏松模型。培养正常和骨质疏松状态下大鼠颌骨及股骨来源的骨髓间充质干细胞。观察细胞形态，进行MTT和CCK-8实验比较四组干细胞的增殖能力。制备四组干细胞膜片与TCP复合进行大网膜移植，术后6w行组织学观察。 结果 去卵巢组BMSCs在同等时间条件下与未去卵巢组比较，初期增殖速度无显著性差异，3d后增殖速度则慢于健康组(P<0.05)。颌骨和股骨BMSCs在健康骨质环境下，细胞的OD值各时间段均未出现统计学差异；骨质疏松状态下颌骨来源BMSCs后期的增殖能力明显优于股骨来源的BMSCs(P<0.05)；四种膜片的大网膜移植结果显示体内移植6w后在的TCP诱导下均可形成不均质的矿化物沉积和纤维样组织穿通，似牙周膜/牙骨质复合体样结构；去卵巢组和未去卵巢组颌骨来源BMSCs组较同等条件下股骨来源BMSCs再生矿化物能力强，牙骨质/牙周膜样复合体的形成更满意。结论 骨质疏松对BMSCs的增殖和分化可能存在着部位因素影响且具有一定的时间依赖性。 |
| 英文摘要: |
| Objective To observe the proliferative capacity and osteogenic differentiation of the bone marrow mesenchymal stem cells from the mandible and the femur of ovariectomized rats. Methods The osteoporotic model was established in female SD rats by ovariectomy. BMSCs were extracted from the mandible (mBMSCs) and the femur (fBMSCs). Cell morphology was observed. MTT and CCK-8 tests were performed to evaluate the cell proliferative capacity. Four kinds of cell sheets were fabricated and transplanted into the greater omentum with TCP block for 6 weeks to evaluate the osteogenic capacity. Result BMSCs from the osteoporotic rats showed the lower growth tendency along with the culture duration compared to the healthy controls. The OD values from the mBMSCs showed the higher proliferative capacity compared to that of the fBMSCs in osteoporotic and healthy group (P<0.05). In vivo transplantation results of the cell sheets fabricated by the four kinds of cells also showed that the cell sheet of mBMSCs could regenerate more periodontium/cemtum-like structure compared to the cell sheet of fBMSCs and cell sheets of osteoporotic group. Conclusion Osteoporosis could down-regulate the biological activity of BMSCs with time- and location-dependence. |
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