中国骨质疏松杂志

骨质疏松症患者肠道菌群多样性分析

Diversity analysis of intestinal microbial flora in osteoporosis patients

DOI：10.3969/j.issn.1006.7108.2017.06.004

英文关键词:Osteoporosis Gut intestinal microbial flora 16S rRNA Sequencing

基金项目:国家自然科学基金（81601898);西安市红会医院科研基金资助课题（YJ2016013);中国博士后科学基金资助项目(2017M613176，2017M613177)

作者	单位
王飙1 赵和平1 高文杰1 李鑫2 梅洋2 乔蓉1 骞雨1 王冀邯1*	1.西安交通大学医学院附属红会医院，陕西西安710054 2.电信科学技术第十研究所，陕西西安710000

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中文摘要:

目的探讨原发性骨质疏松患者肠道菌群的多样性变化。方法收集原发性骨质疏松患者7例与健康对照组7例的粪便样本，提取肠道菌群DNA,16S rRNA基因扩增，Illumina平台测序。对测序结果进行物种注释、多样性分析、物种差异分析。结果原发性骨质疏松患者肠道菌群在纲、目、科、属、种、分类操作单位水平的菌群种类要多于对照组,且两组人群在纲、目、科、属、种、分类操作单位水平的肠道菌群种类有一定的重叠性；Alpha多样性指标中的赵氏指数及艾森指数在两组间差异具有统计学意义（P<0. 05)，香农指数及辛普森指数在两组间差异无统计学意义（P >0.05) ;Beta多样性中的层级聚类能够将两组样本进行鉴别区分；骨质疏松组与对照组样本在门、科、种分类水平的部分肠道菌群含量存在差异(P <0. 05),芽单胞菌门、绿弯菌门、酸杆菌门、芽单胞菌科、丛毛单胞菌科、厌氧绳菌科、毛螺菌、优杆菌等肠道菌群在骨质疏松患者中所占比例要大于正常对照组（P<0.05)。结论 16S rRNA测序有助于分析原发性骨质疏松患者的肠道菌群多样性变化，为研究肠道菌群与骨质代谢的关系提供理论依据。

英文摘要:

Objective To explore the diversity of intestinal microbial flora in osteoporosis patients. Methods Fresh fecal samples from osteoporosis patients (n = 7) and normal controls (n = 7) were collected for DNA extraction, 16S rRNA amplification and Illumina sequencing. Species annotation, diversity and species differences were analyzed based on the sequencing data. Results Compared with normal controls，the intestinal microbial flora species in osteoporosis patients were greater in class, order，family， genus, species and operational taxonomic units (OTUs) levels, and there was certain overlap between the two groups. Chaol estimator and ace estimator of alpha diversity were significantly different between the two groups (P < 0. 05), while shannon index and simpson index showed no significant differences (P >0.05). Hcluster of beta diversity from the sequencing data could identify osteoporosis patients from normal controls. The flora species proportion was significant different between osteoporosis patients and normal controls in phylum, family and species level (P <0.05). Proportions of Gemmatimonadetes, Chlorobacteria, Acidobacteria, Gemmatimonadaceae，Comamonadaceae, Anaerolineaceae, Lachnospira multiparus, Optimal coli，etc. in osteoporosis patients were larger than those in normal controls (P <0. 05). Conclusion 16S rRNA sequencing is a powerful tool for intestinal flora diversity analysis, which can provide theoretical foundation for the correlation study of bone metabolism and intestinal flora.

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