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| 温肾固疏方调控去卵巢大鼠氧化应激及骨代谢指标的机制研究 |
| The mechanism of the warming kidney and astringing formula in oxidative stress and bone metabolism in ovariectomized rats with osteoporosis |
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| DOI：10.3969/j.issn.1006.7108.2017.06.008 |
| 中文关键词: 温肾固疏方 绝经后骨质疏松症 去卵巢大鼠 氧化应激 骨代谢 |
| 英文关键词:The warming kidney and astringing formula Postmenopausal osteoporosis Ovariectomized rats Oxidative stress Bone metabolism |
| 基金项目:江苏省研究生科研创新计划项目(KYZZ15-0272) |
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| 中文摘要: |
| 目的 观察温肾固疏方对去卵巢骨质疏松模型大鼠氧化应激及骨代谢指标的影响。方法 通过切除大鼠双侧卵巢建立绝经后骨质疏松症模型。造模后,将40只模型大鼠随机分为温肾固疏方高、中、低剂量组、雌二醇组、模型组；假手术组仅切除卵巢旁脂肪组织。干预2月后，检测骨组织形态学改变，测定骨密度，采用ELISA法测定血清PINP、BGP、TRACP5b、 RANKL、OPG的含量。比色法检测血清中MDA、CAT、SOD水平。结果 与假手术组比较，模型组股骨近端和股骨总BMD均明显降低，模型组血清MDA活力明显升高（P<0. 05),而CAT活力下降明显（P <0,01),血清TRACP5b、BGP、PINP含量明显升高（P<0.01)，而OPG、OPG/sRANKL比值下降（P<0. 05)，骨形态改变与骨质疏松症一致；与模型组相比：各治疗组骨组织形态改善。雌激素组、温肾固疏方高、中剂量组BMD均明显增高（P<0.01)。雌激素组、温肾固疏高、中剂量组血清PINP、 BGP含量下降（P<0.01)。雌激素组、温肾固疏中剂量组TRACP5b含量下降（P<0.01)。温肾固疏中、低剂量组血清OPG含量升高（P<0.01)。温肾固疏高、中剂量组OPG/sRANKL比值升高（P<0.01)，低剂量组比值明显升高（P<0.01);雌激素组、温肾固疏中剂量组血清MDA活力降低（P<0.01)，温肾固疏中、高剂量组血清CAT活力升高（P<0.01)。结论 温肾固疏方可降低骨代谢高转换水平,增加骨形成，减少骨吸收而发挥抗骨质疏松作用，抗氧化应激机制可能在其中发挥了作用。 |
| 英文摘要: |
| Objective To observe the effect of the warming kidney and astringing formula on oxidative stress and bone metabolism of ovariectomized rats with osteoporosis. Methods Postmenopausal osteoporosis model was established in SD rats by using ovariectomized (OVX). After successful modeling, 40 rats were randomly divided into model group, estradiol valerate group, high-, middle-, and low-dose of the warming kidney and astringing formula groups，and sham group. Rats in the sham group were resected of adipose tissue only. The changes of bone mineral density (BMD)，bone histomorphometry, procollagen type I amino-terminal propeptide (PINP)，tartrate resistant acid phosphatase (TRACP5b), bone gamma-carboxygluta,mic-acid-containing proteins (BGP), receptor activator of NF-kappa B ligand (sRANKL), serum osteoprotegerin (OPG)，superoxide (SOD)，malondialdehyde (MDA),and catalase (CAT) were detected respectively after 2 months. Results Compared to those in sham operation group, BMD of the proximal and the total femur in the model group decreased significantly. Serum MDA, TRACP5b, BGP, and PINP levels in rats of model group increased（P<0.01)，but serum CAT, OPG, and OPG/sRANKL decreased （P<0.01). Compared with the model group, the bone tissue morphology in each treatment group improved. BMD of the proximal and the total femur in estrogen group, the high- and middle-dose of the warming kidney and astringing formula group was significantly higher（P<0.01). Serum OPG，CAT，and OPG/sRANKL levels in rats of the warming kidney and astringing formula groups improved （P<0.01), but serum TRACP5b， BGP, PINP, and MDA decreased（P<0.01). Conclusion The warming kidney and astringing formula inhibits bone resorption, promotes bone formation, and corrects bone metabolism imbalance，which is beneficial to the treatment of postmenopausal osteoporosis. The antioxidant mechanism may play a role in the treatment process. |
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