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| 异补骨脂素对去卵巢大鼠骨代谢的影响研究 |
| Effects of isopsoralen on bone metabolism in ovariectomized rats |
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| DOI：10.3969/j.issn.1006.7108.2017.06.022 |
| 中文关键词: 异补骨脂素 中医中药 大鼠 去卵巢 骨形成 骨质疏松模型 |
| 英文关键词:Isopsoralen Chinese traditional medicine Rats Ovariectomy Bone formation Osteoporosis model |
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| 中文摘要: |
| 目的 研究异补骨脂素对去卵巢大鼠骨代谢的影响。方法 取3月龄雌性SD大鼠40只，随机分为4组，假手术对照组，模型组，雌二醇组（苯甲酸雌二醇0.2 mg/kg，皮下注射，每周1次），异补骨脂素组。手术6 w后，异补骨脂素组用25 mg/ kg(此浓度经过前期浓度筛选得到）异补骨脂素灌胃，每天1次，1周停止1次，模型组灌胃与用药组等体积生理盐水，假手术组正常饲养。12 w后双能X骨密度仪检测全身骨密度后处死，分离骨组织，于AG-X系列台式电子万能试验机检测左侧股骨和椎骨生物力学指标，血清中骨钙素（osteocalcin,OC)和抗酒石酸性磷酸酶5b(lartrale resistanl acid phosphatase 5b，TRACP 5b)检测，右侧股骨护骨素（osteoprotegerin,OPG) ,RANKL免疫组织化学染色，剥离心、肝、脾、肺、肾、肾上腺和子宫称重，计算器官指数，并做常规病理学检测。结果 与假手术组比较，模型组大鼠体质量、全身骨密度、股骨骨密度、腰椎骨密度及血清 OC、TRACP5b，胫骨微组织结构和股骨生物力学性能差异极显著，有统计学意义(P<0.01)，说明骨质疏松造模良好；与模型组比较，异补骨脂素组大鼠体质量、全身骨密度、股骨骨密度、腰椎骨密度及血清OC、TRACP5b，胫骨微组织结构和股骨生物力学性能显著差异有统计学意义（0. 01< P<0. 05)。与模型组比较，阳性对照组大鼠全身与股骨骨密度、血清OC、TRACP5b 和股骨生物力学性能极显著差异有统计学意义（P<0.01）。药物处理组与阳性对照相比无统计差异。结论 25 mg/kg的异补骨脂素能增加SD大鼠骨密度，改善骨组织微结构，有效提升骨生物力学性能，促进骨形成，降低骨质疏松骨折发生的风险。 |
| 英文摘要: |
| Objective To study the effects of isopsoralen on bone formation in ovariectomized rats. Methods Forty female SD rats were randomly divided into four groups ： sham-operation control group，model group，estradiol group (estradiol benzoate 0.2 mg/kg, subcutaneously once a week), and isopsoralen group. After 6 weeks of operation, the rats in the isopsoralen group were treated with 25 mg/kg (this concentration after pre-concentration of screening) isopsoralen once a day, and rest once in a week. The rats in the model group were given the same volume of normal saline. Rats in the sham operation group was fed normally. After 12 weeks, bone mineral density was measured using dual-energy X-ray absorptiometry. The bone biomechanical parameters of the left femur and vertebrae were measured with AG-X series. The serum osteocalcin (OC) and tartrate resistant acid phosphatase 5b (TRACP) were measured. Osteoprotegerin ( OPG) and RANKL were immunohistochemically stained on the right femur. The liver, spleen, lung, kidney，adrenal gland, and uterus were weighed for the calculation of organ index, and the routine pathological examination was performed. Results Comparing to those in the sham group, the body weight, whole body bone mineral density, femoral bone mineral density, lumbar vertebra bone mineral density, serum OC and TRACP-5b, tibial microstructure and biomechanical properties in the model group were significantly different (P <0. 01) , indicating that osteoporosis model was well established. Comparing to those in the model group，the body mass，whole body bone mineral density，bone mineral density of the femurs and lumbar vertebra, serum OC and TRACP-5b, tibial microstructure, and femoral biomechanical properties were significantly different (0.01 |
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