| Objective To investigate the characteristics of genes expression profiles of primary osteoporosis with kidney yang deficiency through analyzing the gene expression difference with gene micro-array. Methods Patient with osteoporosis were rand omly divided into kidney-yang deficiency group (n =3) , kidney-yin deficiency group (n =3)，non-kidney deficiency group (n = 3) , according to the syndrome differentiation of traditional Chinese medicine. Another 3 people with normal BMD were selected as normal control. Expression profiles of the bone tissue from 4 groups were detected to screen differentiated expression genes. Analysis of pathway and other function among these genes was conducted. Results The number of differentiated expression genes in kidney-yang deficiency group were 3976, 6184，and 2631，compared to kidney-yin deficiency, non-kidney deficiency, and normal control group, respectively. The number of common differentially expressed genes were 1037. These genes were involved in 22 pathways, including complement and coagulation cascades, Hedgehog signaling pathway, TGF-beta signaling pathway, and cell cycle. Conclusion Genes related to kidney-yang deficiency syndrome in primary osteoporosis are mainly related to complement and coagulation cascades,Hedgehog signaling pathway, TGF-beta signaling pathway, and cell cycle. |