|
| 去卵巢骨质疏松大鼠骨组织肥大细胞增生及其病理学意义 |
| Mast cell proliferation in the bone tissue of ovariectomized osteoporotic rats and its pathological significance |
| |
| DOI：10.3969/j.issn.1006.7108.2017.07.005 |
| 中文关键词: 大鼠 去卵巢 骨质疏松 肥大细胞 |
| 英文关键词:Rat Ovariectomy Osteoporosis Mast cell |
| 基金项目:陕西省科技创新团队项目（2012KCT-29);陕西省科协青年人才托举项目（20160128);陕西理工大学大学生创新创业项目（2017037);陕西理工大学博士后项目（SLGBH16-03) |
|
| 摘要点击次数: 636 |
| 全文下载次数: 540 |
| 中文摘要: |
| 目的 观察肥大细胞在去卵巢骨质疏松大鼠骨组织中的分布，探讨肥大细胞增生与绝经后骨质疏松症之间的关系。 方法 3月龄健康雌性SD大鼠20只，随机分为假手术组（Sham组）和去卵巢组（OVX组），卵巢去除后3个月，采集和分离血清样品测定血清雌二醇、碱性磷酸酶（alkaline phosphatase, ALP)、骨保护素（osteoprotegerin, OPG)和核因子κB受体活化因子配体（receptor activator of NF-κB ligand，RANKL)，收集股骨近端干骺端、胫骨近端干骺端组织样品进行常规组织切片制作和肥大细胞的甲苯胺蓝染色，并计数单位面积肥大细胞的数量。结果 OVX组大鼠血清雌二醇、OPG含量较Sham组显著降低（P <0.01)，血清ALP、RANKL含量较Sham组显著增高（P <0. 01);组织化学染色结果显示肥大细胞主要分布于大鼠胫骨、股骨骨髓腔中，OVX大鼠胫骨、股骨骨髓腔肥大细胞数量较Sham组显著增多（P< 0.01〉。结论 3月龄大鼠卵巢去除后3个月呈现骨质疏松症状，骨髓腔肥大细胞数量与骨质疏松呈正相关，可作为绝经后骨质疏松症诊断和治疗的依据。 |
| 英文摘要: |
| Objective To investigate the distribution of mast cells in the bone tissue of ovariectomized osteoporotic rats, and to explore the relationship between mast cell proliferation and postmenopausal osteoporosis. Methods Twenty 3-month-old healthy female SD rats were rand omly divided into sham operation group ( Sham group) and ovariectomized group (OVX group). After 3 months of operation, the serum samples were collected to detect the levels of estradiol，alkaline phosphatase ( ALP)， osteoprotegerin { OPG)，and receptor activator of NF-κB ligand (RANKL). The femoral metaphysis and proximal metaphysis were collected to make histological sections. Mast cells were stained with toluidine blue. The number of mast cells were counted per unit area. Results The levels of serum estradiol and OPG in the OVX group decreased significantly compared with those in the Sham group (P <0. 01). The levels of serum ALP and RANKL were higher than those in the Sham group (P < 0. 01) . Histochemical staining showed that mast cells were mainly distributed in bone marrow cavity of the tibia and femur. The number of mast cells in the tibia and femoral bone marrow cavity in the OVX group was higher than that in the Sham group (P <0. 01). Conclusion Osteoporosis symptom appears in 3 month after ovariectomy in 3-month-old rats. The number of mast cells in the bone marrow cavity is positively correlated with osteoporosis, which can be used as the basis for the diagnosis and treatment of postmenopausal osteoporosis. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |