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| 不同紫外线辐射剂量率对大鼠血清25-( OH) D水平及皮肤效应的影响 |
| The influence of different ultraviolet (UV) exposure frequency on serum 25 (OH) D and skin in rats |
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| DOI：10.3969/j.issn.1006.7108.2017.07.007 |
| 中文关键词: 紫外线 暴露频率 25羟基维生素D 皮肤损伤 大鼠 |
| 英文关键词:Ultraviolet(UV) Exposure frequency 25( OH) D Skin damage Rat |
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| 中文摘要: |
| 目的 探讨在紫外线总暴露剂量相同情况下，多次小剂量暴露与集中大剂量暴露对生长期大鼠血清25-(OH)D和皮肤效应的影响。方法 选用90只21 d断乳雄性wistar大鼠，分为1MED组（27只）、1/3MED组（27只）、1/5MED组（27只）及避 光组（9只）4组。饲以维生素D缺乏饲料。1/5MED组大鼠连续照射15 d,每日照射剂量为1/5MED; 1/3MED组实验期内间断照射9 d，每天照射剂量为1/3MED;1MED组实验期内间断照射3 d，每天照射量为1MED;实验期内累计暴露剂量均为 3MED。避光组接受白炽灯照射，实验期内累计暴露剂量为0 mj/cm2。实验期为15 d,测定各组血清25-(OH)D水平，观察皮肤改变情况。结果 4组大鼠血清25-(OH)D浓度差异明显（F = 20.695，P<0.001)。其中1/5MED组的血清25-( OH) D浓度为（151.97±73.52) nrnol/L，高于 1/3MED 组的（136. 21 ± 71. 39) nmol/L,但差异无统计学意义（P = 0. 966 ) ; 1/5MED、1/ 3MED组均显著高于1MED组（54.50 ±20. 52)nmol/L和避光组（15. 63 ±0. 82)nmol/L，差异有统计学意义（P <0, 001)；1MED 组高于避光组（P <0.001)。1/5MED组在照射5次后背部皮肤开始出现轻度红斑、无水肿；1/3MED组在照射3次后开始出现中度红斑，轻度水肿；1MED组大鼠在照射1次后开始出现重度红斑，中度水肿；而对照组在实验期间未出现红斑，水肿。 结论 紫外线暴露能够促进大鼠内源性维生素D的合成，多次小剂量暴露可明显提高大鼠血清25-(OH)D水平，且皮肤损伤明显减轻。 |
| 英文摘要: |
| Objective To investigate the effect of multiple exposure with low-dose UV or concentrated high-dose UV exposure under same amount of total dose on serum 25 (OH) D and the skin in growing rats. Methods Ninety 21-day weaning male Wistar rats were divided into 1MED group (n= 27)，1/3MED group (n = 27)，1/5MED group (n =27)，and incand escent lamp group (n=9). Rats were fed with vitamin D deficiency meal. The rats in 1/5MED group were irradiated with 1/5MED UV dose per day for consecutive 15 days. The rats in 1/3MED group were intermittently irradiated with 1/3MED UV dose for 9 days. The rats in 1MED group were intermittently irradiated for 3 days with a 1 MED daily dose of UV. The cumulative exposure dose was 3 MED in all groups except the incand escent lamp group during the experimental period. Rats in incand escent lamp group was exposed to incand escent lamp only，and the cumulative UV exposure dose was 0 mj/cm2. The plasma level of 25 (OH) D was measured and the change of skin was observed after the 15-day experimental period in each group. Results The serum 25 (OH) D concentrations were significantly different among the 4 groups (F = 20. 695，P <0. 001) . The serum 25(OH)D in 1/5MED group (151. 97 ± 73. 52 nmol/L) was higher than that in the 1/3MED group (136. 21 ±71.39 nmol/L). There was no significant difference between the two groups (P = 0. 96). The serum 25(OH)D in 1/5MED and 1/3MED was significantly higher than that in 1 MED group (54. 50 ±20, 52 nmol/L) and incand escent lamp group (15. 63 ±0. 82 nmol/L, P <0. 001). The serum 25(OH)D in 1MED group was higher than that in incand escent lamp group (P <0. 001). The skin of rats began to appear mild erythema, with no edema after 5 times irradiation in the 1/5MED group. It began to appear moderate erythema, edema after 3 times irradiation in 1/3MED group and began to appear the severe erythema and moderate edema after 1 time irradiation in the 1 MED group. Rat in incand escent lamp group did not appear erythema and edema during the trial. Conclusion Ultraviolet exposure can promote the synthesis of endogenous vitamin D in rats. Multiple small-dose UV exposure is better in increase of serum 25(OH)D and reducing skin damage. |
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