|
| 卵巢切除大鼠在不同时期RANKL、OPG蛋白表达的变化及与BTMs的相关性 |
| Protein expression of RANKL and OPG at different periods in ovariectomized rats and their relationships with bone turnover markers |
| |
| DOI：10.3969/j.issn.1006-7108.2017.08.001 |
| 中文关键词: 绝经后骨质疏松 骨转换标志物 骨保护素 核因子κB受体活化因子配体 |
| 英文关键词:Postmenopausal osteoporosis Bone turnover markers Osteoprotegerin (OPG) NF-κB Ligand (RANKL) |
| 基金项目:国家自然科学基金资助项目(81574003)；福建省科技厅引导性项目(2015Y0069)；福建省自然科学基金计划资助项目(2014J01355)；福建省中医药科技项目(wzgs201309) |
|
| 摘要点击次数: 1386 |
| 全文下载次数: 631 |
| 中文摘要: |
| 目的 探讨卵巢切除大鼠在不同时期骨保护素(OPG)、核因子κB受体活化因子配体(RANKL)的表达及与骨转换标志物（BTMs）的关系。方法 将SD雌性大鼠随机分成假手术组（Sham）和去卵巢骨质疏松模型组（OVX），模型组行双侧卵巢切除术，术后第2、6、10、14周分批取材，采用Western blot检测各时期大鼠骨组织OPG、RANKL蛋白表达，采用Elisa检测各时期大鼠血清骨转换标志物BGP、BALP、CTX-I、TRAP-5b的变化。结果 ①OVX组在术后第2周(P<0.01)和第10、14周(P<0.05)骨组织RANKL蛋白表达均较Sham组显著升高，OPG蛋白表达在第2周(P<0.01)和第6周(P<0.05)均显著降低。②与Sham组相比，OVX组术后第2周血清BGP、BALP、CTX-I(P<0.05)和TRAP-5b(P<0.01)水平均显著升高；OVX组术后第6周血清BGP、BALP水平显著升高(P<0.01)，CTX-I(P<0.05)和TRAP-5b(P<0.01)水平显著下降；OVX组术后第10、14周血清CTX-I(P<0.05)、BALP(P<0.01)水平显著升高。③骨转换标志物血清CTX-I、TRAP-5b水平与骨OPG表达成负相关(P<0.01)，与骨RANKL表达成正相关(P<0.05)。结论 相关性分析显示骨转换标志物CTX-I、TRAP-5b与骨OPG成负相关，与骨RANKL成正相关。 |
| 英文摘要: |
| Objective To explore the relationship between bone turnover markers (BTMs) and protein expression of NF-κB Ligand (RANKL) and Osteoprotegerin (OPG) at different periods in ovariectomized rats. Methods SD rats were randomly divided into two groups: sham operated group (Sham) and ovariectomy group (OVX). OVX group had bilateral ovariectomy. Samples were taken from rats at 2, 6, 10 and 14 weeks after operation. Serum levels of bone alkaline phosphatase (BALP), bone gla protein (BGP), Carboxy-terminal telopeptide of type I collagen (CTX-I), tartrate-resistant acid phosphatase 5b (TRAP-5b) were detected using ELISA method. Protein expression levels of OPG and RANKL were measured using Western blot method. Results (1) Protein expression of RANKL in the OVX group was higher than that in the Sham group at 2 weeks (P<0.01) and at 10 and 14 weeks (P<0.05) after operation, while OPG was lower at 2 weeks (P<0.01) and 6 weeks (P<0.05) after operation. (2) Compared with the Sham group, the serum levels of BGP, BALP, CTX-I (P<0.05) and TRAP-5b (P<0.01) in the OVX group increased significantly at 2 weeks after operation. The serum levels of BGP and BALP (P<0.01) in the OVX group increased significantly, while CTX-I (P<0.05) and TRAP-5b (P<0.01) levels decreased significantly at 6 week after operation. The serum levels of CTX-I (P<0.05) and BALP (P<0.01) in the OVX group increased significantly at 10 and 14 weeks after operation. (3) The serum levels of BTMs CTX-I and TRAP-5b negatively correlated with the protein expression of OPG (P<0.01), and positively correlated with the protein expression of RANKL (P<0.05). Conclusion Correlation analysis showed that the serum level of BTMs CTX-I and TRAP-5b were negatively correlated with the protein expression of OPG, and positively correlated with the protein expression of RANKL. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |