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| 唑来膦酸、聚乳酸-羟基乙酸聚合物、β-磷酸三钙复合支架修复去势大鼠股骨干骺端骨缺损的实验研究 |
| Experimental study of zoledronic acid / poly (lactic-co-glycolic acid) / β-tricalcium phosphate composite scaffold on the repair of femoral metaphyseal defect in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2017.08.003 |
| 中文关键词: 唑来膦酸 聚乳酸-羟基乙酸聚合物 β-磷酸三钙 去势大鼠 骨缺损 |
| 英文关键词:Zoledronic acid Poly (lactic-co-glycolic acid) β-tricalcium phosphate Ovariectomized rats Bone defect |
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| 中文摘要: |
| 目的 唑来膦酸、聚乳酸-羟基乙酸聚合物、β-磷酸三钙(ZA、PLGA、β-TCP) 复合支架对去势大鼠股骨干骺端骨缺损的修复作用。方法 雌性SD大鼠经过双侧去卵巢手术后饲养3个月建立骨质疏松模型，随后在大鼠双侧股骨干骺端建立直径为3mm 圆形骨缺损，上述大鼠随机分为4 组；分别置入ZA、PLGA支架，β-TCP支架和ZA、PLGA、β-TCP复合支架，不置入支架材料的为对照组。术后12 周取材，通过Micro-CT 扫描重建和病理组织学评价三组支架材料的成骨作用。结果 术后观察发现空白对照组骨缺损未修复，骨缺损断端硬化，而其他 3 组骨缺损均有不同程度修复。植入骨质疏松大鼠体内12周后，三组材料随着材料的降解均有新生骨长入，且三组新骨生成率均显著优于对照组(P ＜0. 05)，缺损区域都有较高BV/TV、Tb.Th、Tb.N 、Conn. D、骨矿化沉积率(MAR)和较低的Tb.Sp，其中以ZA、PLGA、β-TCP组最高。Micro-CT 和病理组织学结果提示 ZA、PLGA、β-TCP组骨修复效果较 ZA、PLGA与β-TCP组更好。结论 ZA、PLGA、β-TCP复合支架具有明显促进去势大鼠股骨干骺端骨缺损修复的作用 |
| 英文摘要: |
| Objective To study the effect of zoledronic acid / poly (lactic-co-glycolic acid) / β-tricalcium phosphate (ZA / PLGA / β-TCP) composite scaffold on the repair of femur metaphyseal defect in ovariectomized rats. Methods Ovariectomized female SD rats were fed for 3 months to establish osteoporosis model, then round bone defect of 3 mm diameter was established in bilateral femur metaphysis of rats. The rats were randomly divided into 4 groups. The ZA/PLGA scaffold was implanted through open operation into the bone defects in Group ZA/PLGA, Group β-TCP was implanted with β-TCP scaffold, Group ZA/PLGA/β-TCP was implanted with ZA/PLGA/β-TCP composite scaffold, and the control group did not have any implant. Twelve weeks after operation, osteogenesis was evaluated by Micro-CT scanning and histopathological evaluation. Results Observation found no repair but sclerosis of bone ends in the control group while bone repair of different extents was observed in the other 3 groups. Twelve weeks after the implantation, compared with the control group, the three implanted group had significantly greater new bone formation rate (P <0.05), and higher BV / TV, Tb.Th, Tb.N, Conn. D and bone mineralization deposition rate (MAR) and lower Tb.Sp at the defect areas, with the highest observed in the ZA / PLGA / β-TCP group. Micro-CT and histopathological results showed that the ZA / PLGA / β-TCP group had better bone repair than the ZA / PLGA and the β-TCP groups. Conclusion ZA / PLGA / β-TCP composite scaffolds can significantly improve the repair of femur metaphyseal bone defects in ovariectomized rats. |
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