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| 葛根素对过氧化氢致成骨细胞氧化损伤的影响 |
| The effect of puerarin on the damage of osteoblasts induced by hydrogen peroxide |
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| DOI：10.3969/j.issn.1006.7108.2017.11.008 |
| 中文关键词: 葛根素 成骨细胞 过氧化氢 |
| 英文关键词:Puerarin Osteoblast Hydrogen peroxide |
| 基金项目: |
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| 中文摘要: |
| 目的 研究具有抗氧化作用的植物雌激素——葛根素（puerarin，Pue)对氧化应激后成骨细胞（osteoblast，OB)的影响。方法 采用酶多次消化法，由出生24 h以内的Wistar大鼠头盖骨分离OB，培养、传代及诱导分化、矿化等。用0.1 mmol/L H2O2制造氧化应激状态模型。将细胞随机分为对照组、H2O2组[H'组]、“Pue(10-10mol/L~10-3mol/L) + H2O2”组[“P + H”’组]和“H2O2 +Pue(10-10mol/L~10-3mol/L)”组[“H' +P”组]。观察不同处理组细胞的增殖、分化功能，并测定其微量丙二醛（mMDA)含量、超氧化物歧化酶（super oxide dismutase，SOD)活性、总抗氧化能力（T-AOC)等。结果 与H2O2组相比，“P + H”’组和“H' +P”组中Puel0-7mol/L ~ 10-4mol/L使细胞的增殖、分化功能，SOD活性和T-AOC均有显著升髙（P <
0.01)，而mMDA含量则显著降低（P <0. 01)。结论 H2O2过量会导致OB的氧化损伤，而葛根素在体外对H2O2介导OB的氧化损伤具有保护作用。 |
| 英文摘要: |
| Objective To investigate the effect of a potent anti-oxidative phytoestrogen，puerarin，on osteoblasts under oxidative stress. Methods Osteoblasts were isolated from the calvarias of new-born Wistar rats with enzyme digestion method and cultured. The cells of the first to third generation were randomly divided into 4 groups: the control group，the 10-4mol/L H2O2 group，the 10 -4mol/L H2O2 + 10 -5 mol/L puerarin group，and the 10 -5 mol/L puerarin group. The proliferation and differentiation of osteoblasts were determined. The content of maleic dialdehyde (MDA)，the activity of superoxide dismutase (SOD)，and total anti-oxidative capability (T-AOC) were tested and compared. Results Compared with those in the H2O2 group，the proliferation and differentiation of osteoblasts，the activity of SOD，and T-AOC in the model groups increased significantly (P <0.01). However，the content of MDA decreased significantly (P <0. 01). Conclusion Over-dose of H2 O2 can cause oxidative damage in osteoblasts. Puerarin can prevent osteoblasts from oxidative damage in vitro. |
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