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| 类黄酮对人体外周血破骨细胞分化p38MAPK/c-Fos信号通路调控的研究 |
| Regulation of flavonoids on p38MAPK/c-Fos signaling pathway in human peripheral blood osteoclast differentiation |
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| DOI：10.3969/j.issn.1006.7108.2017.11.018 |
| 中文关键词: 类黄酮 破骨细胞 信号通路 细胞分化 |
| 英文关键词:Flavonoid Osteoclast Signal pathway Cell differentiation |
| 基金项目:陇原青年创新人才扶持计划 |
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| 摘要点击次数: 883 |
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| 中文摘要: |
| 目的 探讨类黄酮调控p38MAPK/c-Fos信号通路对破骨细胞分化的影响。方法 采用人体外周血分离单个核细胞，并诱导形成破骨细胞，干预组应用类黄酮对细胞进行处理，通过细胞形态学观察并检测细胞活性，再分别采用RT-PCR和Western blot两种方法观察p38MAPK通路及下游转录因子c-fos基因和蛋白的表达水平。结果 对照组与干预组细胞形态学观察均可见诱导培养的破骨细胞形态明显;与对照组比较，干预组破骨细胞样细胞的形成减少，活性减弱，差异具有统计学意义（P <0.05)；RT-PCR结果显示，干预组p38MAPK通路下游转录因子c-fos表达水平较对照组下降，差异具有统计学意义 (P <0. 01);Western blot检测结果显示，干预组c-fos蛋白表达水平较对照组降低，差异具有统计学意义（P <0.05)。结论 类黄酮在体外细胞培养中可抑制破骨细胞形态，并可通过下调p38MAPK通路中c-fos基因和蛋白水平的表达，从而减弱破骨细胞的吸收功能。 |
| 英文摘要: |
| Objective To investigate the effect of flavonoids on p38MAPK/c-Fos signaling pathway in osteoclast differentiation. Methods Using human peripheral blood mononuclear cells，osteoclasts were induced. Cells in the intervention group received flavonoids treatment. Cell morphology and cell activity were detected. Expression of genes and proteins of the p38MAPK pathway and the downstream transcription factor c-fos was observed using RT-PCR and Western blotting，respectively. Results Cell morphology of osteoclast in the control group and the intervention group showed obvious osteoclast morphology after induction. Compared with the control group，osteoclast like cell formation and activity in the intervention group decreased，and the difference was statistically significant (P <0.05). RT-PCR result showed that the expression levels of p38MAPK downstream transcription factor c-fos was lower in the intervention group than that in the control group，and the difference was statistically significant (P < 0.01). Western -blot assay showed that the expression level of c-fos protein in intervention group was lower than that in the control group，and the difference was statistically significant (P <0. 05). Conclusion Flavonoids inhibits osteoclast morphology in vitro and reduces the osteoclast absorption function by down regulating the expression of c-fos and protein in p38MAPK pathway. |
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