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| 藤黄健骨胶囊对去卵巢骨质疏松大鼠骨密度和骨代谢的影响 |
| Effect of gamboge strong-bone capsule on bone mineral density and bone metabolism in ovariectomized rats |
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| DOI：10.3969/j.issn.1006.7108.2018.01.002 |
| 中文关键词: 藤黄健骨胶囊 骨质疏松 骨密度 骨代谢 |
| 英文关键词:Gamboge strong-bone capsule Osteoporosis Bone mineral density Bone metabolism |
| 基金项目:甘肃省高等学校科研项目（2015A-097）；甘肃中医药大学教学研究与教学改革项目(ZH-201606)；甘肃省高校重大疾病分子医学与中医药防治研究重大实验室开放基金(FZYK15-16-7) |
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| 中文摘要: |
| 目的 研究藤黄健骨胶囊对骨质疏松大鼠骨密度和骨代谢的影响。方法 采用去卵巢法制备大鼠骨质疏松症模型，灌胃藤黄健骨胶囊干预，灌胃 1个月后取材。称重法检测各组大鼠的体重，酶联免疫吸附(ELISA)试剂盒检测血清骨钙素（osteocalcin，BGP）和抗酒石酸酸性磷酸酶（tartrate resistant acid phosphatase，trACP）含量；双能X线吸收测量仪测量获得骨密度（bone mineral density，BMD）；AG-IX生物力学万能实验机检测最大载荷和断裂载荷；HE染色法观察股骨的病理形态学变化。结果 与假手术组比较，模型组大鼠体重明显升高，血清抗酒石酸酸性磷酸酶明显升高（P<0.05）；而模型组大鼠血清骨钙素明显降低，骨密度明显减少，最大载荷和断裂载荷明显减少（P<0.05）；模型组骨小梁结构不完整、丢失、断裂严重。与模型组相比，藤黄健骨胶囊组大鼠血清骨钙素的含量和抗酒石酸酸性磷酸酶的活性得到纠正（P<0.05），股骨的骨微观参数骨密度均得到了良好地修复，骨生物力学参数中最大载荷、断裂载荷得到了明显修复（P<0.05），骨小梁排列规则，数目增多、增宽。结论 藤黄健骨胶囊能够修复去卵巢骨质疏松症大鼠股骨微观结构破坏和股骨生物力学性能的下降，提示藤黄健骨胶囊具有良好的抗骨质疏松作用。 |
| 英文摘要: |
| Objective To explore the effect of gamboge strong-bone capsule on bone mineral density (BMD) and bone metabolism in osteoporotic rats. Methods Rats were ovariectomized to establish the osteoporosis model. Gamboge strong-bone capsule was used for intervention. The samples were harvested in one month. The body weight of rats in each group was measured. BGP and trACP were measured using ELISA method. BMD was examined with dual energy X ray absorptiometer. The maximum load and fracture load were determined with AG-IX biomechanical universal testing machine. The morphological changes of the femur were observed with HE staining. Results Compared with those in the sham group, the weight and trACP content raised obviously in the model rats (P<0.05). In model rats, BGP and BMD reduced significantly (P<0.05), the maximum load and fracture load declined (P<0.05), and the trabecular bone structure was incomplete, missing, and broken seriously. Compared with those in the model group, BGP and trACP were corrected in gamboge strong-bone capsule group, trabecular microstructure was well repaired, the maximum strength and break strength significantly improved, bone trabecula was arranged regularly, and bone trabecula increased in number and width (P<0.05). Conclusion Gamboge strong-bone capsule greatly improves the trabecular microstructure and the biomechanical properties of the femur in osteoporotic rat model. It may have great potential in the prevention of osteoporosis. |
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