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| Wnt/β-catenin、RANKL/LGR4通路因子在骨质疏松性骨折端的表达 |
| The expression of factors of Wnt/β-catenin and RANKL/LGR4 pathways in osteoporotic fractures |
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| DOI：10.3969/j.issn.1006.7108.2018.03.008 |
| 中文关键词: 骨质疏松性骨折 Wnt/β-catenin通路 RANKL/LGR4通路 关键因子 |
| 英文关键词:Osteoporotic fracture Wnt/β-catenin signaling pathway RANKL/LGR4 signaling pathway Key factors |
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| 中文摘要: |
| 目的 探讨骨质疏松性骨折（osteoporotic fracture，OPF）骨折端Wnt/β-catenin、RANKL/LGR4通路关键因子的表达。方法 选取2016年1月至2017年7月在我院就诊的符合标准的股骨骨折患者56例，分为OPF组27例和对照组29例，伤后3~7d行骨折手术，于骨折断面内用刮匙刮取骨组织≥200mg，保存于液氮中。使用液氮研磨RNAiso Plus法提取骨组织总RNA，采用RT-PCR技术检测比较两组之间各因子mRNA的表达差异；使用液氮研磨加裂解液提取骨组织总蛋白，采用Western blotting技术检测比较两组之间各因子蛋白的表达差异。结果 RT-PCR、Western blotting检测OPF组对比对照组患者LRP5、β-catenin、Runx2、C-myc、LGR4因子表达均降低（P＜0.05），RANKL因子表达升高（P＜0.05）。结论 OPF与Wnt/β-catenin成骨信号通路中LRP5、β-catenin、Runx2、C-myc因子成骨抑制或减弱有关，与RANKL/LGR4破骨信号通路中RANKL因子破骨增强、LGR4因子破骨抑制有关。可通过调控各因子的表达干预OPF的发生和治疗。 |
| 英文摘要: |
| Objective To detect the expression of key factors of Wnt/β-catenin and RANKL/LGR4 pathways in fracture tissues of patients with osteoporotic fracture (OPF). Methods Fifty-six patients with femoral fractures from January 2016 to July 2017 in our hospital were selected. Twenty-seven cases were divided into OPF group and 29 cases were divided into control group. Surgery was performed in 3-7 days. Bone tissue (more than 200mg) from the fracture area was collected and stored in liquid nitrogen. Total RNA was extracted from bone tissue using liquid nitrogen grinding RNAiso Plus method. The difference of mRNA expression of each factor was compared between the two groups using RT-PCR technique. Total protein was extracted from bone tissue using liquid nitrogen grinding and pyrolysis liquid. The protein expression difference of each factor was detected and compared between the two groups using Western blotting technique. Results RT-PCR and Western blotting results showed that LRP5, β-catenin, Runx2, C-myc, and LGR4 decreased, but RANKL increased in OPF group, comparing to those in the control group (P<0.05). Conclusion OPF is associated with the inhibition or weakening of osteogenic factors LRP5, β-catenin, Runx2, and C-myc in Wnt/β-catenin osteogenic signaling pathway, the increase of osteoclastic factor RANKL in RANKL/LGR4 osteoclastic signaling pathway, and the inhibition of osteoclastic factor LGR4. The occurrence and treatment of OPF can be intervened by regulating the expression of various factors. |
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