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| ERK5信号通路介导流体剪切力对MC3T3-E1成骨细胞MMPs、TIMPs表达的影响 |
| ERK5 signaling pathway mediates the effects of fluid shear stress on the expression of MMPs and TIMPs in MC3T3-E1 osteoblasts |
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| DOI：10.3969/j.issn.1006.7108.2018.05.006 |
| 中文关键词: 流体剪切力 MC3T3-E1 细胞 细胞外信号调节激酶5 基质金属蛋白酶 基质金属蛋白酶抑制剂 |
| 英文关键词:Fluid shear stress MC3T3- E1 cell Extracellular signal-regulated kinase 5 Matrix metalloproteinase Matrix metalloproteinase inhibitory factor |
| 基金项目:国家自然科学基金（81672207）；甘肃省青年科技研究基金项目（1506RJYA233） |
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| 中文摘要: |
| 目的 观察流体剪切力（fluid shear stress，FSS）作用下，MC3T3-E1 成骨细胞中细胞基质金属蛋白酶（matrix metalloproteinases，MMPs）和基质金属蛋白酶抑制剂（tissue inhibitors of metalloproteinases，TIMPs）的表达情况，并探讨 ERK5 信号通路在其中的作用。方法 对MC3T3-E1成骨细胞进行不同的处理，分为正常组、XMD8-92组 、FSS组和FSS+XMD8-92 组，对FSS组施加 12 dyn /cm2 流体剪切力，采用蛋白免疫印迹法分别检测P-ERK5、ERK5、 MMPs和TIMPs 蛋白水平的变化。结果 生理强度（12 dyn/cm 2 ）的流体剪切力作用于MC3T3-E1成骨细胞45min后能显著上调MMPs的表达，下调TIMPs的表达，但此效应可被 ERK5 高选择性抑制剂 XMD8-92 阻断。结论 ERK5 信号通路调控流体剪切力对成骨细胞MMPs、TIMPs蛋白的表达。 |
| 英文摘要: |
| Objective To observe the expression matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in MC3T3-E1 osteoblastic cells induced by fluid shear stress, as well as to study the effect of ERK5 signaling pathway in it. Methods MC3T3-E1 cells were treated with FSS or XMD8-92 alone, or in combination, and were subjected to fluid shear stress (12dyn /cm2) for 0, 15, 30, 45, 60 minutes. The protein levels of extracellular signal-regulated kinase 5 (ERK5), phospho-ERK5 (P-ERK5), MMPS and TIMPs were detected by Western blotting. Results For MC3T3-E1 cells cultured in vitro, loading of 12 dyn /cm2 fluid shear stress for 45 minutes could up regulate the expression of MMPs, down regulate the expression of TIMPs, but the effect was blocked by XMD8-92 (a highly selective inhibitor of ERK5 activity). Conclusions ERK5 signaling pathway plays essential roles in the expression of MMPs and TIMPs induced by fluid shear stress in the MC3T3-E1 osteoblastic cells. |
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