中国骨质疏松杂志

全身振动训练对绝经大鼠骨骼肌的改善及其作用机制研究

Study on the mechanism of whole-body vibration training on skeletal muscles in ovariectomized rats

DOI：10.3969/j.issn.1006.7108.2018.05.011

中文关键词: 全身振动训练绝经大鼠骨骼肌 β-连环蛋白 GSK-3β PPARγ

英文关键词:Whole body vibration training Ovariectomized rat Skeletal muscle β-catenin GSK-3β PPARγ

基金项目:

作者	单位
张轲* 罗由平	宜宾市第二人民医院小儿外科，四川宜宾 644000

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中文摘要:

目的通过观察全身振动性训练法（WBV）对去卵巢（OVX）大鼠骨骼肌中过氧化物酶体增殖剂激活受体（PPARγ），β-连环蛋白和磷酸化糖原的表达合成酶激酶-3（P-GSK-3β）蛋白质表达的影响，探讨WBV对绝经大鼠骨骼肌的作用机制。方法将雌性大鼠随机分成正常对照组（Sham组，n = 10），去卵巢组（OVX组，n = 10），17β-雌二醇治疗组（E2组，n = 10）和全身振动性训练组（WBV组，n = 10）。测定各组大鼠血清E2和黄体生成素（LH）水平，以及PPARγ、β-连环蛋白和P-GSK-3β在骨骼肌中的表达。结果 OVX组大鼠血清LH比Sham组和E2组明显升高（P<0.05）。OVX组大鼠血清E2水平与Sham组、E2组和WBV组相比明显上升（P<0.05）。OVX组骨骼肌PPARγ表达较Sham组显著升高（P < 0.05），而β-连环蛋白和P-GSK-3β蛋白表达较Sham组均显著降低（P < 0.05）。在WBV或雌激素替代治疗的干预下，WBV组或E2组的PPARγ表达均显著低于OVX组（P<0.05），β-连环蛋白和P-GSK-3β蛋白表达均显著高于OVX组（P<0.05）。结论 WBV能有效预防OVX大鼠的骨质流失，并提高骨质强度，这可能与抑制OVX大鼠骨骼肌中GSK-3β和PPARγ活性来激活β-连环蛋白信号传导通路有关。

英文摘要:

Objective To explore the mechanism of whole body vibration training (WBV) on skeletal muscles of postmenopausal rats, through the observation of expressions of β-catenin signaling through phosphorylation of GSK-3β and peroxisome proliferators-activated receptor γ (PPARγ) in the skeletal muscles of ovariectomized (OVX) rats. Methods Rats were randomly divided into the sham operated group (Sham group, n=10), castrated control group (OVX group, n=10), 17 β-estradiol treatment group (E2 group, n=10), and whole-body vibration training group (WBV group, n=10). At the end of the experiment, the serum levels of estradiol (E2) and luteinizing hormone (LH), and protein expressions for PPARγ, β-catenin, and P-GSK-3β in skeletal muscles were examined. Results The level of serum LH in OVX group was higher than that in Sham group and E2 group (P<0.05). The level of serum E2 in OVX group was lower than that of other 3 groups (P<0.05). Compared with Sham group, the expression of PPARγ in skeletal muscles of OVX group increased significantly (P<0.05), while the expressions of β-catenin and P-GSK-3β protein decreased significantly (P<0.05). Under the intervention of WBV or estrogen replacement therapy, the expression of PPARγ in WBV group or E2 group was significantly lower than that in OVX group (P<0.05), but the expressions of β-catenin and P-GSK-3β protein were significantly higher than those in OVX group (P<0.05). Conclusion WBV activates the GSK-3β/β-catenin signaling but inhibits the production of PPARγ in skeletal muscles of OVX rats, which may contribute to the prevention of bone loss in OVX rats.

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