| Objective To study the effect of Bcl2 and Bak1 recombinant adenovirus alone or in co-transfection on the cell activity and related proteins of MG63 cells. Methods Logarithmic MG63 cells were collected and divided into 4 groups: Group A was non-virus-load control group, B and C groups were transfected with Bcl2 and Bak1 recombinant adenovirus, respectively, and group D was Bcl2 and Bak1 recombinant adenovirus co-transfection group. After each group was treated with corresponding treatment, MTT assay was used to detect cell proliferation, and the activity of alkaline phosphatase (ALP) was determined using the ALP assay kit. Flow cytometry was used to detect Ca2+ concentration, and the expression of connective tissue growth factor (CTGF), osteopontin (OPN), Runx2 and tumor necrosis factor-α (TNFα) was detected by Western blot. Results ① Compared with group A, the cell activity, ALP activity and the relative expression of CTGF, OPN and Runx2 increased, while Ca2+ concentration and the relative expression of TNFα decreased, and the activity of ALP decreased (P<0.05) in group B. The cell activity and ALP activity decreased, and Ca2+ concentration increased in group C and the relative expression of proteins were opposite to those of group B (P<0.05). There were no significant differences between group D and group A (P>0.05). ② There were significant differences between groups B, C and D (P<0.05). Conclusion Bcl2 recombinant adenovirus transfected MG63 cells could enhance cell activity and calcification, and promote bone formation. |