| Objective To explore the characteristics of gene expression profiles and bioinformatics in postmenopausal osteoporosis patients with Kidney Yin deficiency. Methods Patients with postmenopausal osteoporosis were randomly chosen and according to TCM syndrome differentiation assigned into kidney yin deficiency (n=3) and kidney yang deficiency groups (n=3), and three healthy postmenopausal women were selected as control group. MicroRNA gene expression in single nuclear cells of peripheral blood was examined using microarray method. Patients in the kidney yin deficiency group were compared with the other two groups. The common differential expression genes were selected and the dysregulated miRNAs were detected by qRT-PCR for further confirmation of microarray data. The differential expression of miRNA was analysed by bioinformatics methods including pathway analysis and target gene prediction. Results There were 20 common differential expression genes. The relative expression level of hsa-miR-411-5p, hsa-miR-143-3p and hsa-miR-95-3p was consistent with the microarray data. The pathway analysis showed that these differential expression genes were involved in metabolic pathways, in cancer, Rap1 signaling pathway, focal adhesion, pi3k-akt signaling pathway, MAPK signaling pathway, calcium signaling pathway, protein processing in endoplasmic reticulum, CGMP-PKG signaling pathway, Ras signaling pathway and WNT signaling pathway. At last we screened 9 target genes (RC3H1, SOX11, FUT4, GABRA4, NUFIP2, ONECUT2, PHF20, PURB and ZNF148). Conclusion MiRNA expression profiling of postmenopausal osteoporosis patients with Kidney Yin deficiency was established successfully. The 20 differential expressions of miRNAs involved in postmenopausal osteoporosis with Kidney Yin deficiency by regulation of PI3K-Akt, MAPK and Wnt signaling pathway. |