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| 3T3-E1细胞增殖分化过程中雷洛昔芬对内质网应激的影响 |
| Effect of raloxifene on endoplasmic reticulum stress during the proliferation and differentiation of 3T3-E1 cells |
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| DOI：10.3969/j.issn.1006-7108.2018.06.015 |
| 中文关键词: 成骨细胞 雷洛昔芬 细胞增殖 内质网应激 骨质疏松，绝经后 |
| 英文关键词:Osteoblast Raloxifene Cells proliferation Endoplasmic reticulum stress Osteoporosis, postmenopausal |
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| 中文摘要: |
| 探讨选择性雌激素受体调节剂(selective estrogen receptor modulator，SERM)雷洛昔芬对3T3-E1细胞增殖分化的作用及其机制。方法 用不同浓度的雷洛昔芬（10-9 mol/L、10-8 mol/L、10-7 mol/L）处理培养3T3-E1细胞，另设空白对照组，24 h后检测各指标。CCK8法检测细胞增殖，使用碱性磷酸酶测定试剂盒测定碱性磷酸酶（alkaline phosphatase，ALP）活力，实时荧光定量聚合酶链式反应(real time quantitative polymerase chain reaction，qRT-PCR)检测细胞内IRE-1、ATF6、eIF2α的表达。结果 雷洛昔芬与对照组相比较可促进3T3-E1细胞增殖（P＜0.05），随着雷洛昔芬浓度的增高，细胞增殖程度升高（P＜0.05），高浓度的雷洛昔芬（10-7 mol/L）促增殖能力最强；雷洛昔芬不同浓度处理后各组ALP活力增加（P＜0.05），而且高浓度雷洛昔芬（10-7 mol/L）升高ALP活力较明显；IRE-1、ATF6及eIF2α的表达均升高（P＜0.05）。结论 雷洛昔芬可能通过上调IRE-1、ATF6及eIF2α的表达促进3T3-E1细胞的增殖分化。 |
| 英文摘要: |
| Objective To explore the role and mechanism of selective estrogen receptor modulator -raloxifene on the proliferation and differentiation of the 3T3-E1 cells. Methods 3T3-E1 cells were cultured with different concentrations of raloxifene (10-9 mol/L, 10-8 mol/L and 10-7 mol/L), and a blank control group was also established. Indicators were measured after 24 hours. Cell proliferation was detected with cell counting kit-8 (CCK8), ALP activity was determined using the ALP kit, and the expression of IRE-1, ATF6 and eIF2α were measured by the qRT-PCR. Results Compared with the control group, raloxifene can promote the proliferation of 3T3-E1 cells (P<0.05). With the increase of raloxifene concentration, the proliferation of the 3T3-E1 cells increased (P<0.05), with the highest concentration of raloxifene (10-7 mol/L) had the strongest effect. The ALP activity of the 3T3-E1 cells increased after treatment with raloxifene, and the effect was most evident at the highest concentration of raloxifene. The mRNA expression of IRE-1,ATF6 and eIF2α increased in with raloxifene treatment (P＜0.05). Conclusion Raloxifene could promote the proliferation and differentiation of 3T3-E1 cells through up-regulating the mRNA expression of IRE-1, ATF6 and eIF2α. |
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