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| ERK5在小鼠骨质疏松性骨折愈合过程中作用的实验研究 |
| Study of ERK5 in the healing process of osteoporotic fracture in mice |
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| DOI：10.3969/j.issn.1006.7108.2018.07.003 |
| 中文关键词: ERK5 骨质疏松 骨折 成骨相关蛋白 动物实验 |
| 英文关键词:ERK5 Osteoporosis Fracture Osteogenesis-related protein Animal experimentation |
| 基金项目:国家自然科学基金（81672207）；兰州大学第二医院博士科研基金（ynbskyjj2015-2-9） |
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| 中文摘要: |
| 目的 通过构建小鼠骨质疏松骨折模型，研究ERK5在骨质疏松性骨折愈合过程中的作用。方法 将108只6周龄雌性昆明小鼠随机分成4组，通过手术切除小鼠双侧卵巢及小鼠股骨离断分别构建骨质疏松（OVX）和骨折模型（Fracture），然后给实验组小鼠每日腹腔注射ERK5特异性阻断剂XMD8-92，于1 w、2 w、4 w后分别处死一定数量的小鼠，取术侧股骨标本，行X线片检查、股骨骨痂Micro-CT、HE染色及免疫组织化学染色检查，观察骨折端骨小梁生长情况，骨痂内成骨相关蛋白及ERK5表达情况。结果 给予实验小鼠注射XMD8-92后第2周及第4周，Fracture组小鼠骨痂生长较快，骨小梁数目较多，厚度较大，成骨相关蛋白ALP、Runx2的表达相对较多（P＜0.05），而Fracture+XMD8-92组小鼠骨痂生长则相对缓慢，骨小梁稀少且绯薄，结构相对较乱，碱性磷酸酶(alkaline phosphatase，ALP)、Runx2表达较少（P＜0.05）；且OVX+Fracture+XMD8-92组小鼠与OVX+Fracture组小鼠相比，骨小梁生成更少且紊乱，骨痂生长明显延迟，ALP、Runx2表达量显著减少（P＜0.05）。结论 ERK5影响骨折端骨痂形成的速度和质量，在促进骨质疏松性骨折愈合过程中起着十分重要的生理作用。 |
| 英文摘要: |
| Objective To study the role of ERK5 in the healing process of osteoporotic fractures by establishing osteoporotic fracture model in mice. Methods 108 6-week-old female mice were randomly divided into 4 groups. Osteoporosis and fracture models were established by surgically resecting the bilateral ovaries and cutting off the femur. Then the experimental mice were injected with ERK5-specific blocker XMD8-92 intraperitoneally, and a certain number of mice were sacrificed at the 1st, 2nd and 4th week. X-ray examination, Micro-CT, HE staining and immunohistochemically staining of bone callus were performed to observe the growth of trabecular bone, and the expression of osteogenesis-related proteins and ERK5. Results The experimental mice were injected with XMD8-92 at the 2nd week and the 4th week. In the fracture group, the callus of mice grew faster, there were more and thicker trabecular bone, and the expression of the osteogenesis-related proteins ALP and Runx-2 was higher (P<0.05). The callus grew much slower with less and thinner trabecular bone, and the expression of the osteogenesis-related proteins ALP and Runx-2 was lower in the fracture+XMD8-92 group (P<0.05). Compared with the osteoporotic fracture group, the osteoporotic fracture + XMD8-92 group showed less and more disordered trabecular bone formation, significant delay in the growth of callus, and significantly lower ALP and Runx-2 expression (P<0.05). Conclusion ERK5 affects the rate and quality of callus formation in the area of fracture and plays an important physiological role in the process of osteoporotic fracture healing. |
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