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| 阿托伐他汀钙对成骨前体细胞（3T3-E1）增殖与分化的影响及其与内质网应激的联系 |
| Effect of atorvastatin on proliferation and differentiation of osteoblast cells （3T3-E1） and its relationship with endoplasmic reticulum stress |
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| DOI：10.3969/j.issn.1006.7108.2018.07.014 |
| 中文关键词: 阿托伐他汀钙 成骨前体细胞 细胞增殖 内质网应激 |
| 英文关键词:Atorvastatin calcium Osteoblast cells (3T3-E1 cells) Cell proliferation Endoplasmic reticulum stress |
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| 中文摘要: |
| 目的 观察不同浓度阿托伐他汀钙对成骨前体细胞(3T3-E1细胞)增殖与分化影响，分析该过程与内质网应激相关基因Bip、PERK、Eif2a表达的关系。方法 3T3-E1细胞培养至对数期，加入含不同浓度阿托伐他钙0、10-8、10-7、10-6mol/L培养液继续培养细胞，24 h后利用CCK-8比色法检测细胞增殖能力，碱性磷酸酶测定试剂盒测定ALP活力，实时定量PCR技术检测内质网应激标志性基因Bip、PERK、Eif2a表达水平。结果 ①镜下观察3T3-E1细胞呈梭形或多角形等形态，生长状态良好。②与对照组相比，不同浓度的阿托伐他汀钙作用于细胞24h后，均可促进3T3-E1细胞增殖并增加ALP活性（P＜0.05），其中以10-6mol/L阿托伐他钙浓度增殖最明显。③实时定量PCR结果显示，阿托伐他汀钙组Bip、PERK、Eif2a表达均高于对照组（P＜0.05）。结论 阿托伐他汀钙可促进3T3-E1细胞增殖，促进细胞ALP活性增加，提高内质网应激标志性基因表达，提示阿托伐他汀促进增殖的作用可能与内质网应激通路相关，为骨质疏松的临床治疗及药物研发提供新的思路。 |
| 英文摘要: |
| Objective To observe the proliferation and differentiation of atorvastatin calcium with different concentrations on the 3T3-EI cells and to analyze the relationship between this process and the expression of endoplasmic reticulum stress related genes, Bip, PERK, and Eif2a. Methods The osteoblast cells were cultured to the logarithmic phase, and then the cells were continued to be cultured with different concentrations of atopal calcium in 0, 10-8, 10-7, 10-6mol/L.After 24 hours，the cell proliferation ability was detected by CCK-8 colorimetric method，the activity of ALP was measured by alkaline phosphatase assay，and the expression level of symbolic endoplasmic reticulum genes were detected by the real time quantitative PCR technique. Results ① Under microscope, cells were spindle or polygonal. ②Compared with the control group，atorvastatin calcium could promote cell proliferation and increase the activity of ALP (P < 0.05), and proliferation was most obvious in 10-6mol/L. ③Real time quantitative PCR showed that the expressions of Bip, PERK and Eif2a in atorvastatin calcium group were higher than those in the control group (P < 0.05). Conclusion Atorvastatin can promote the proliferation of 3T3-E1 cells and increase the activity of ALP cells.The fact which atorvastatin can promote the proliferation may be related to the endoplasmic reticulum stress pathway, which provides a new method for the clinical treatment and drug development of osteoporosis. |
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