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| 慢性间歇性缺氧对大鼠骨代谢及OPG、RANKL基因表达的影响 |
| The effect of chronic intermittent hypoxia on bone metabolism and mRNA expression of OPG and RANKL in rats |
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| DOI：10.3969/j.issn.1006-7108.2018.08.007 |
| 中文关键词: 慢性间歇缺氧 骨代谢 骨保护素 核因子κ B受体活化因子配体 |
| 英文关键词:Chronic intermittent hypoxia Bone metabolism OPG RANKL |
| 基金项目:江西省青年科学基金资助项目-慢性间歇性缺氧对骨代谢的影响及可能机制（20161BAB215235） |
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| 中文摘要: |
| 目的 通过建立wistar大鼠慢性间歇性缺氧(chronic intermittent hypoxia ，CIH)模型探讨CIH对骨
代谢及骨组织骨保护素(osteoprotegerin,OPG)、核因子κ B受体活化因子配体（receptor activator for nuclear factor-κ B ligand，RANKL）基因表达的影响。 方法 20只健康wistar大鼠随机分为正常对照组及CIH组，每组10只。对照组置于空气循环舱内，CIH组于置于间歇性缺氧舱内，每日8 h，共8周。应用双能X线骨密度仪测定大鼠全身、股骨、腰椎骨密度(bone mineral density, BMD)，采用ELISA法检测大鼠血清骨特异性碱性磷酸酶(bone specific alkaline phosphatase,BALP)、骨钙素( bone glaprotein,BGP)、I型胶原N端前肽（type I collagen N terminal peptide ,PINP）、I型胶原羧基端肽β降解产物(β-C-terminal telopeptide of type I collagen,β-CTX)、抗酒石酸酸性磷酸酶(tartrate resistant acid phosphatase ,TRAP)水平，应用Real-time PCR技术测定股骨OPG、RANKL mRNA相对表达量。结果 与对照组相比，CIH组全身和股骨BMD下降[(0.141±0.028) vs （0.122±0.027）和（0.143±0.026 ）vs（0.125±0.034)]、血清BGP降低[(26.42±3.71) vs (22.05±2.16) ]、血清β-CTX和TRAP升高[(132.24±26.25) vs (173.82±22.16)和(20.12±2.43 )vs (23.58±2.36)]、股骨组织OPG mRNA 相对表达量降低[(1.031±0.125) vs (0.924±0.141)] 、RANKL mRNA相对表达量升高[(0.856±0.068) vs (1.113±0.134)]，差异均具有统计学意义(P<0.05)。结论 慢性间歇性缺氧可使骨组织OPG mRNA表达减少、RNAKL mRNA表达增加，影响骨代谢，降低骨密度，增加了骨质疏松的发生风险。 |
| 英文摘要: |
| Objective To investigate the effect of chronic intermittent hypoxia (CIH) on bone metabolism and the mRNA expression of OPG and RANKL in bone tissue using Wistar rat CIH model. Methods 20 healthy Wistar rats were randomly divided into normal control group and CIH group, with 10 rats in each group. The control group was placed in the air circulation cabin, and the CIH group was placed in the intermittent hypoxic capsule for 8 hours per day for 8 weeks. Bone mineral density (BMD) of whole body, femur and lumbar spine were determined using DXA. Serum levels of rat bone specific alkaline phosphatase (BALP), osteocalcin (BGP), type I collagen N terminal peptide (PINP), type I collagen carboxy-terminal peptide, beta degradation products (beta-CTX), tartrate resistant acid phosphatase (TRAP) of rats were measured by ELISA. Real-time PCR was applied to determinate the mRNA expression of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B ligand (RANKL) in the femur of rats. Results Compared with the control group, the CIH group’s BMD of whole body and femur decreased [(0.141±0.028) vs (0.122±0.027) and (0.143±0.026) vs (0.125±0.034)], serum BGP decreased [(26.42±3.71) vs (22.05±2.16)], serum beta-CTX and TRAP increased [(132.24±26.25) vs (173.82±22.16) and (20.12±2.43) vs (23.58±2.36)], mRNA expression of OPG in the femoral tissue decreased [(1.031±0.125) vs (0.924±0.141)], while mRNA expression of RANKL increased [(0.856±0.068) vs (1.113±0.134)]. These differences were all statistically significant (P<0.05). Conclusion Chronic intermittent hypoxia might reduce OPG mRNA, increase RNAKL mRNA of bone tissue, affect bone metabolism, reduce bone mineral density and increase the risk of osteoporosis. |
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