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| 衰老骨髓间充质干细胞中端粒短缩与成骨能力下降相关性研究 |
| The abbreviation of telomere and the decline of osteogenic differentiation in senescent BMSCs: A correlation study |
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| DOI：10.3969/j.issn.1006-7108.2018.10.011 |
| 中文关键词: 骨髓间充质干细胞 衰老 端粒 成骨分化 动物实验 |
| 英文关键词:Bone marrow mesenchymal stem cells Senescence Telomere Osteogenic differentiation Animal experiment |
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| 中文摘要: |
| 目的 骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）是组织工程中修复骨和软骨缺损的重要种子细胞。老年供体BMSCs的增殖速度和分化能力均低于年轻供体，其相关机制仍不清楚，希望通过相关实验对其进行探究。方法 采用SD大鼠的BMSCs进行培养，并通过应用H2O2处理和连续传代诱导细胞衰老。通过β-半乳糖苷酶染色和端粒长度分析检测衰老，同时对细胞增殖和成骨分化能力进行检测。应用Olaparib维持端粒长度来研究端粒长度在细胞衰老、增殖和分化能力方面的影响。结果 H2O2可以增加BMSCs中β-半乳糖苷酶染色阳性率、缩短端粒长度、降低细胞增殖率和碱性磷酸酶活性。BMSCs长期传代后也可观察到成骨分化的衰老。抑制端粒长度下降可降低H2O2诱导所致的β-半乳糖苷酶染色阳性率增加，促进细胞增殖，增强成骨分化能力。结论 端粒长度下降可引起BMSCs的衰老，从而降低其成骨分化能力、抑制干细胞增殖。 |
| 英文摘要: |
| Objective Bone marrow mesenchymal stem cells (BMSCs) is an important seed cell for the repair of bone and cartilage defect in the tissue engineering. The proliferation rate and differentiation capacity of BMSCs from the old donors were less than that from young donors; however, the related mechanism remained unclear. Methods Sprague Dawley (SD) rats BMSCs were cultured and treated. Hydrogen peroxide (H2O2) and continual passage of BMSCs were performed to induce senescence. Senescence was detected by the SAβ-Gal staining and the telomere length analysis. Cell proliferation and osteogenic differentiation were also observed. Olaparib was used to maintain the telomere length and investigate the role of telomere length and senescence on the cell proliferation and differentiation. Results H2O2 could increase the positive rate of SA-β-Gal staining in BMSCs and shorten the length of the telomere. The proliferation rate and ALP activity were also decreased by the H2O2. The senescence and decline of osteogenic differentiation could also be observed after prolonged passage of BMSCs. Inhibition of telomere length decline could attenuate the increased positive rate of SA-β-Gal staining induced by the H2O2, promote the cell proliferation, and enhance the capacity of osteogenic differentiation. Conclusion Senescence can be induced by the decline of telomere length and could reduce the capacity of osteogenic differentiation and inhibit cell proliferation in BMSCs. |
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