中国骨质疏松杂志

雷洛昔芬联合仙灵骨葆对骨质疏松骨微结构和生物力学性能的影响

The effect of raloxifene and Xianlinggubao on the micro-structure and biomechanical property of osteoporotic bone

DOI：10.3969/j.issn.1006.7108.2018.11.007

英文关键词:raloxifene Xianlinggubao osteoporosis trabecula bone biomechanics collagen fiber proteoglycan

基金项目:

作者	单位
王丽随1 范哲华2 陈海英2*	1.福建莆田市第一医院药剂科，福建莆田 351100 2. 福建莆田学院，福建莆田 351100

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中文摘要:

目的探讨雷洛昔芬联合仙灵骨葆对骨质疏松大鼠骨微结构及其生物力学性能的影响。探讨其调控骨吸收与骨形成及其防治骨质疏松症的作用机制。方法 6月龄40 只SD大鼠在骨质疏松造模之后，随机分为正常组、假手术组、模型组、干预1组、干预2组，每组8只。造模手术1周后，干预1组采用经口灌胃雷洛昔芬6.2 mg/(kg.d)，干预2组采用经口灌胃雷洛昔芬6.2 mg/(kg.d)+仙灵骨葆250 mg/(kg.d)，而正常组、假手术组、模型组采用经口灌胃注射用水。12周后应用组织病理学分析及组织计量学测定；利用Van Gieson胶原纤维染色法观察胶原纤维沉积变化；ELISA 测定血清蛋白聚糖活性。分离右侧股骨进行三点弯曲试验，检测股骨生物力学性能。结果对照组骨小梁粗厚、有完整的成骨细胞区。模型组骨小梁稀疏细薄、细胞核固缩；与对照组相比，模型组胶原纤维明显减少，蛋白聚糖含量明显增加，最大载荷(FM)下降，差异均有统计学意义（均P＜0.05）。干预组骨小梁排列较紧密。干预2组较干预1组，胶原纤维明显增加，蛋白聚糖活性降低，最大载荷(FM)差异具有统计学意义( P＜0.01)。结论雷洛昔芬联合仙灵骨葆较雷洛昔芬单独用药可以显著改善骨质疏松大鼠的骨基质代谢，促进成骨，显著加强骨生物力学性能。

英文摘要:

Objective To investigate the effects of raloxifene combined with Xianlinggubao on bone microstructure and biomechanical property of osteoporotic rats, and to explore its mechanism of regulation of bone resorption and bone formation and its prevention and treatment of osteoporosis. Methods Forty 6-month SD rats were randomly divided into normal group, sham operation group, model group, intervention group 1, and intervention group 2 after osteoporosis modeling. One week after model operation, the rats in intervention group 1 received oral raloxifene 6.2 mg / kg.d, and rats in the intervention group 2 received oral raloxifene 6.2 mg / kg.d plus Xianlinggubao 250 mg / kg.d. Rats in the normal group, the sham operation group, and the model group received water gavage. After 12 weeks, histopathological analysis and histomorphometry were performed. The deposition of collagen fibers was observed with van Gieson collagen fiber staining. The activity of serum proteoglycan activity was determined with ELISA. The right femur was separated for a three-point bending test to examine the biomechanical properties of the femur. Results The control group had thick trabecular bone and a complete osteoblastic area. The trabecular bone in the model group was sparse and thin, and the nucleus was pyknosis. Compared with the control group, the collagen fibers in the model group significantly decreased, the proteoglycan content significantly increased, and the maximum load (FM) decreased. The difference was statistically significant (both P<0.05). The trabecular bone in the intervention group arranged closely. Compared with the intervention group 1, the collagen fibers in the intervention group 2, increased significantly, the proteoglycan activity decreased, and FM was significantly different (P<0.01). Conclusion Compared with raloxifene alone, raloxifene combined with Xianlinggubao can significantly improve the bone matrix metabolism, promote osteogenesis, and significantly enhance the biomechanical properties of the bone in rats with osteoporosis.

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