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| 缺氧诱导因子抑制剂对去卵巢大鼠骨质疏松症治疗效果的研究 |
| Effect of hypoxia inducible factor inhibitor on osteoporosis in ovariectomized rats |
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| DOI：10.3969/j.issn.1006-7108.2018.12.009 |
| 中文关键词: 骨质疏松症 缺氧诱导因子抑制剂 去卵巢 骨密度 骨强度 |
| 英文关键词:osteoporosis hypoxia-inducible factor inhibitor ovariectomy bone mineral density bone strength |
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| 中文摘要: |
| 目的 研究缺氧诱导因子抑制剂对卵巢切除（ovariectomized，OVX）雌性Sprague-Dawley大鼠的治疗效果，比较缺氧诱导因子抑制剂对骨密度的影响。方法 在大鼠卵巢切除12周后，使用缺氧诱导因子抑制剂进行治疗。治疗8周后，在实验结束时将大鼠进行安乐死处理，获取大鼠血清、股骨和胫骨。通过生物力学测试，Micro-CT扫描和血清生化分析进行评估。结果 与未给药的OVX大鼠相比，缺氧诱导因子抑制剂的全身给药显著降低了骨代谢指标Ⅰ型前胶原氨基末端前肽(type I collagen N terminal peptide ，PINP)和Ⅰ型胶原羧基端肽(type I collagen carboxy-terminal peptide ，CTX-I)，增加了大鼠胫骨骨密度（bone mineral density, BMD）,增强了股骨极限载荷、能量和刚度，差异比较有统计学意义（P<0.05）。结论 缺氧诱导因子抑制剂能有效改善OVX诱导的骨质疏松症。 |
| 英文摘要: |
| Objective We investigated the therapeutic effects of the administration of hypoxia inducible factor (HIF) inhibitor in ovariectomized (OVX) female Sprague–Dawley rats and compared the effects on bone mineral density. Methods After 12 weeks of ovariectomy, OVX rats were treated with hypoxia-inducible factor inhibitors. Eight weeks after treatment, rats were euthanized at the end of the experiment to obtain serum, femur and tibia. The effect of these treatments was evaluated by biomechanical tests, Micro-CT scans and serum biochemical analysis. Results Compared with untreated OVX rats, results and statistical analyses showed that systemic administration of HIF inhibitors significantly reduced bone metabolic markers type I procollagen N-terminal propeptide (PINP) and type I collagen carboxyl end peptide (CTX-I), increased rat tibia bone density (BMD) and increased femur load, energy and stiffness, with statistically significant differences (P<0.05). Conclusion Hypoxia-inducible factor inhibitors could effectively improve OVX-induced osteoporosis. |
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